Characterization of blaKPC-3-positive plasmids from an Enterobacter aerogenes isolated from a corvid in Canada

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F18%3AN0000121" target="_blank" >RIV/00027162:_____/18:N0000121 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11140/18:10376553 RIV/62157124:16270/18:43876612 RIV/62157124:16810/18:43876612

Výsledek na webu

<a href="https://academic.oup.com/jac/article-abstract/73/9/2573/5026317?redirectedFrom=fulltext" target="_blank" >https://academic.oup.com/jac/article-abstract/73/9/2573/5026317?redirectedFrom=fulltext</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/jac/dky199" target="_blank" >10.1093/jac/dky199</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Characterization of blaKPC-3-positive plasmids from an Enterobacter aerogenes isolated from a corvid in Canada

Popis výsledku v původním jazyce

To examine the environmental contamination by carbapenemase-producing Enterobacteriaceae (CPE) in Canada, total of 449 faecal samples from wild corvids were analyzed. Carbapenemase production was hypothesized by MALDI-TOF MS meropenem hydrolysis assay. Carbapenemase-encoding genes were detected by PCR. Transfer experiments by conjugation were carried out with Escherichia coli recipients. blaKPC-carrying plasmids were characterized by S1 profiling and Illumina sequencing. Among 242 crbapenem-non-susceptible Gram-negative bacteria, only one KPC-3-producing Enterobacter aerogenes isolate (Eaer-4382) was identified. blaKPC-3 was transferred to E.coli laboratory strain by conjugation, while S1 analysis revealed the presence of two types of transconjugants carrying blaKPC-positive plasmids of different sizes (~90-kb [pEaer-4382s] or ~120-kb [pEaer-4382b]). pEaer-4382b (134521 bp) consisted of an IncFII-type sequence (94320 bp) and an acquired sequence (40201 bp) including a truncated KPC-3-encoding Tn4401b transposon. Plasmid pEaer-4382s (108772 bp) differed by a 25638-bp deletion of the acquired region of pEaer-4382b. Additionally, an 8-bp duplication (ACAAGATA) was identified in the region, between the resD and parA genes, resulting in a different number of iterons. Even only one KPC-3 producer was isolated from a wild corvid, the findings of the present study confirmed the environmental contamination by CPE in Canada. Furthermore, sequencing data underlined the ongoing evolution of blaKPC-carrying mobile elements.

Název v anglickém jazyce

Characterization of blaKPC-3-positive plasmids from an Enterobacter aerogenes isolated from a corvid in Canada

Popis výsledku anglicky

To examine the environmental contamination by carbapenemase-producing Enterobacteriaceae (CPE) in Canada, total of 449 faecal samples from wild corvids were analyzed. Carbapenemase production was hypothesized by MALDI-TOF MS meropenem hydrolysis assay. Carbapenemase-encoding genes were detected by PCR. Transfer experiments by conjugation were carried out with Escherichia coli recipients. blaKPC-carrying plasmids were characterized by S1 profiling and Illumina sequencing. Among 242 crbapenem-non-susceptible Gram-negative bacteria, only one KPC-3-producing Enterobacter aerogenes isolate (Eaer-4382) was identified. blaKPC-3 was transferred to E.coli laboratory strain by conjugation, while S1 analysis revealed the presence of two types of transconjugants carrying blaKPC-positive plasmids of different sizes (~90-kb [pEaer-4382s] or ~120-kb [pEaer-4382b]). pEaer-4382b (134521 bp) consisted of an IncFII-type sequence (94320 bp) and an acquired sequence (40201 bp) including a truncated KPC-3-encoding Tn4401b transposon. Plasmid pEaer-4382s (108772 bp) differed by a 25638-bp deletion of the acquired region of pEaer-4382b. Additionally, an 8-bp duplication (ACAAGATA) was identified in the region, between the resD and parA genes, resulting in a different number of iterons. Even only one KPC-3 producer was isolated from a wild corvid, the findings of the present study confirmed the environmental contamination by CPE in Canada. Furthermore, sequencing data underlined the ongoing evolution of blaKPC-carrying mobile elements.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

30303 - Infectious Diseases

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Antimicrobial Chemotherapy

ISSN

0305-7453

e-ISSN

1460-2091

Svazek periodika

73

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

3

Strana od-do

2573–2575

Kód UT WoS článku

000443539100043

EID výsledku v databázi Scopus

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