Carbapenemase genes in Enterobacteriaceae isolates from wild birds in North America

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16810%2F19%3A43878218" target="_blank" >RIV/62157124:16810/19:43878218 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Carbapenemase genes in Enterobacteriaceae isolates from wild birds in North America

Popis výsledku v původním jazyce

The emergence and global spread of acquired carbapenemase-producing Enterobacterales (CPE) are of great concern for public health. Although resistance to carbapenems is associated mainly with hospital-acquired infections, there is a growing number of evidence of CPE in companion and food-producing animals and the environment including several reports from wildlife. The aim of the study was to examine American crows for CPE and to perform molecular typing using whole-genome sequencing (WGS) as well as to study transferability and closer genetic context of carbapenemase genes. A total of 590 faecal samples collected in five sampling sites across USA were cultivated on MacConkey agar with meropenem (0.125 mg/l) and ZnSO4 (100 mg/l). Two hundred and seventyseven Gram-negative bacteria with reduced susceptibility to meropenem (&gt;0.125 mg/l) were identified by MALDI-TOF, tested for the production of carbapenemase by recently developed HRC assay and the presence of carbapenemase genes (blaKPC, blaOXA-48, blaVIM, blaNDM, blaIMP, blaIMI, blaGES) using PCR followed by sequencing. Clonality of the CPE and plasmid content was determined by pulse-field gel electrophoresis and their susceptibility to antibiotics was tested using disk diffusion method. WGS using Ilumina MiSeq was performed to evaluate presence of antibiotic resistance genes, plasmid replicons and sequence types (ST). The gene transferability into recipient cells was tested by conjugation experiments and Southern blot hybridization was used to verify the gene location. Thirteen CPE isolates including Providencia rettgeri (blaIMP-27, n=11), Klebsiella pneumoniae ST258 (blaKPC-2, n=1) and Enterobacter asburiae (blaIMI-1, n=1) from birds in two locations were detected. P. rettgeri isolates were assigned to two unrelated PFGE clusters and blaIMP-27 was located on the chromosome as a part of class 2 integron. Similar isolates have been recently described in both human and farm animal isolates in USA. In multiresistant K. pneumoniae ST258 isolate, blaKPC-2 was carried by a pKpQIL-like plasmid as commonly found in human clinical isolates. The gene blaIMI-1 in E. asburiae was located within a structure showing &gt;99% nucleotide identity to EcloMEX, an integrative mobile element exploiting the Xer recombinases of the host for integration, described in human clinical isolates from Canada. This study showed further evidence of anthropogenic derived dissemination of plasmid- and chromosomally-encoded carbapenemases into the environment

Název v anglickém jazyce

Carbapenemase genes in Enterobacteriaceae isolates from wild birds in North America

Popis výsledku anglicky

The emergence and global spread of acquired carbapenemase-producing Enterobacterales (CPE) are of great concern for public health. Although resistance to carbapenems is associated mainly with hospital-acquired infections, there is a growing number of evidence of CPE in companion and food-producing animals and the environment including several reports from wildlife. The aim of the study was to examine American crows for CPE and to perform molecular typing using whole-genome sequencing (WGS) as well as to study transferability and closer genetic context of carbapenemase genes. A total of 590 faecal samples collected in five sampling sites across USA were cultivated on MacConkey agar with meropenem (0.125 mg/l) and ZnSO4 (100 mg/l). Two hundred and seventyseven Gram-negative bacteria with reduced susceptibility to meropenem (&gt;0.125 mg/l) were identified by MALDI-TOF, tested for the production of carbapenemase by recently developed HRC assay and the presence of carbapenemase genes (blaKPC, blaOXA-48, blaVIM, blaNDM, blaIMP, blaIMI, blaGES) using PCR followed by sequencing. Clonality of the CPE and plasmid content was determined by pulse-field gel electrophoresis and their susceptibility to antibiotics was tested using disk diffusion method. WGS using Ilumina MiSeq was performed to evaluate presence of antibiotic resistance genes, plasmid replicons and sequence types (ST). The gene transferability into recipient cells was tested by conjugation experiments and Southern blot hybridization was used to verify the gene location. Thirteen CPE isolates including Providencia rettgeri (blaIMP-27, n=11), Klebsiella pneumoniae ST258 (blaKPC-2, n=1) and Enterobacter asburiae (blaIMI-1, n=1) from birds in two locations were detected. P. rettgeri isolates were assigned to two unrelated PFGE clusters and blaIMP-27 was located on the chromosome as a part of class 2 integron. Similar isolates have been recently described in both human and farm animal isolates in USA. In multiresistant K. pneumoniae ST258 isolate, blaKPC-2 was carried by a pKpQIL-like plasmid as commonly found in human clinical isolates. The gene blaIMI-1 in E. asburiae was located within a structure showing &gt;99% nucleotide identity to EcloMEX, an integrative mobile element exploiting the Xer recombinases of the host for integration, described in human clinical isolates from Canada. This study showed further evidence of anthropogenic derived dissemination of plasmid- and chromosomally-encoded carbapenemases into the environment

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů