Providencia rettgeri with IMP-27 carbapenemase from American crows

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16810%2F18%3A43876821" target="_blank" >RIV/62157124:16810/18:43876821 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Providencia rettgeri with IMP-27 carbapenemase from American crows

Popis výsledku v původním jazyce

A total of 277 Gram-negative bacteria, obtained from faecal specimens of American crow (Corvus brachyrhynchos) in USA, with reduced susceptibility to meropenem (&gt;0.125 mg/L) were tested for the presence of carbapenemase encoding gene blaIMP using a set of PCR experiments. Eleven IMP-27-producing Providencia rettgeri isolates were detected and tested for antimicrobial susceptibility, epidemiological and phylogenetic relatedness, transferability and genetic context of blaIMP-27 gene. P. rettgeri isolates showed resistant phenotype to cephalosporins and tetracycline and were assigned to two epidemiological unrelated clusters. Isolates from the first cluster originated from two different regions and they carried lnu(F) gene conferring resistance to lincosamides and a plasmid 40-60 kb in size. All isolates from the second cluster originated from the same location, harboured a 120 kb plasmid and no additional resistance determinants. The gene blaIMP-27 was located on the chromosome as a gene cassette of a class 2 integron. Although the prevalence of carbapenemase producers in this study was low, our results point out that wild birds are possible vectors of bacteria resistant to antibiotics of last resort

Název v anglickém jazyce

Providencia rettgeri with IMP-27 carbapenemase from American crows

Popis výsledku anglicky

A total of 277 Gram-negative bacteria, obtained from faecal specimens of American crow (Corvus brachyrhynchos) in USA, with reduced susceptibility to meropenem (&gt;0.125 mg/L) were tested for the presence of carbapenemase encoding gene blaIMP using a set of PCR experiments. Eleven IMP-27-producing Providencia rettgeri isolates were detected and tested for antimicrobial susceptibility, epidemiological and phylogenetic relatedness, transferability and genetic context of blaIMP-27 gene. P. rettgeri isolates showed resistant phenotype to cephalosporins and tetracycline and were assigned to two epidemiological unrelated clusters. Isolates from the first cluster originated from two different regions and they carried lnu(F) gene conferring resistance to lincosamides and a plasmid 40-60 kb in size. All isolates from the second cluster originated from the same location, harboured a 120 kb plasmid and no additional resistance determinants. The gene blaIMP-27 was located on the chromosome as a gene cassette of a class 2 integron. Although the prevalence of carbapenemase producers in this study was low, our results point out that wild birds are possible vectors of bacteria resistant to antibiotics of last resort

Druh

O - Ostatní výsledky

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů