Carbapenemase-Producing Gram-Negative Bacteria from American Crows in the United States

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000007" target="_blank" >RIV/00027162:_____/21:N0000007 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/21:00122205 RIV/62157124:16170/21:43879602 RIV/62157124:16810/21:43879602 RIV/62157124:16270/21:43879602

Výsledek na webu

<a href="https://aac.asm.org/content/65/1/e00586-20" target="_blank" >https://aac.asm.org/content/65/1/e00586-20</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1128/AAC.00586-20" target="_blank" >10.1128/AAC.00586-20</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Carbapenemase-Producing Gram-Negative Bacteria from American Crows in the United States

Popis výsledku v původním jazyce

This study was focused on wild corvid species as a reservoir of carbapenemase-producing Gram-negative bacteria in the USA. From a total of 590 faecal samples of American crow, eleven Providencia rettgeri isolates harbouring blaIMP-27 gene, an isolate of Klebsiella pneumoniae sequence type (ST) 258 with blaKPC-2 and an isolate of Enterobacter cloacae complex with blaIMI-1 were obtained by selective cultivation on meropenem-supplemented media followed by PCR detection of carbapenemase-encoding genes and whole-genome sequencing. P. rettgeri isolates were assigned to two phylogenetically unrelated clusters harbouring blaIMP-27 gene located on the chromosome as a class 2 integron gene cassette within Tn7 transposon. K. pneumoniae ST258 isolate showed a multi-drug resistance profile and carried blaKPC-2 on a pKpQIL-like plasmid as a part of Tn4401a. The phylogenetic comparison of 237 genomes of K. pneumoniae ST258 obtained from BIGSdb database showed high similarity of the corvid isolate to human isolates. In E. cloacae complex isolate, the gene blaIMI-1 was located within an integrative mobile element exploiting the Xer recombinases, EcloIMEX-2. Despite that we observed the low prevalence (4.7%, n=277) of Gram-negative bacteria with carbapenemase genes in corvids in the USA, the outcome of the study highlights the role of anthropogenic derived dissemination of carbapenemase-producing bacteria to wildlife and raise attention to corvids as possible environmental vector of bacteria resistant to critically important antimicrobials.

Název v anglickém jazyce

Carbapenemase-Producing Gram-Negative Bacteria from American Crows in the United States

Popis výsledku anglicky

This study was focused on wild corvid species as a reservoir of carbapenemase-producing Gram-negative bacteria in the USA. From a total of 590 faecal samples of American crow, eleven Providencia rettgeri isolates harbouring blaIMP-27 gene, an isolate of Klebsiella pneumoniae sequence type (ST) 258 with blaKPC-2 and an isolate of Enterobacter cloacae complex with blaIMI-1 were obtained by selective cultivation on meropenem-supplemented media followed by PCR detection of carbapenemase-encoding genes and whole-genome sequencing. P. rettgeri isolates were assigned to two phylogenetically unrelated clusters harbouring blaIMP-27 gene located on the chromosome as a class 2 integron gene cassette within Tn7 transposon. K. pneumoniae ST258 isolate showed a multi-drug resistance profile and carried blaKPC-2 on a pKpQIL-like plasmid as a part of Tn4401a. The phylogenetic comparison of 237 genomes of K. pneumoniae ST258 obtained from BIGSdb database showed high similarity of the corvid isolate to human isolates. In E. cloacae complex isolate, the gene blaIMI-1 was located within an integrative mobile element exploiting the Xer recombinases, EcloIMEX-2. Despite that we observed the low prevalence (4.7%, n=277) of Gram-negative bacteria with carbapenemase genes in corvids in the USA, the outcome of the study highlights the role of anthropogenic derived dissemination of carbapenemase-producing bacteria to wildlife and raise attention to corvids as possible environmental vector of bacteria resistant to critically important antimicrobials.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Antimicrobial Agents and Chemotherapy

ISSN

0066-4804

e-ISSN

1098-6596

Svazek periodika

65

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

19

Strana od-do

—

Kód UT WoS článku

000600658500005

EID výsledku v databázi Scopus

2-s2.0-85098669102