Detection and characterization of methicillin-resistant Staphylococcus aureus isolated from milk samples of cows, sheep, and goats in the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F18%3AN0000130" target="_blank" >RIV/00027162:_____/18:N0000130 - isvavai.cz</a>

Výsledek na webu

<a href="https://cit.vfu.cz/konference/mvp2018/4download/sbornik.pdf" target="_blank" >https://cit.vfu.cz/konference/mvp2018/4download/sbornik.pdf</a>

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Detection and characterization of methicillin-resistant Staphylococcus aureus isolated from milk samples of cows, sheep, and goats in the Czech Republic

Popis výsledku v původním jazyce

This study aimed to detect and characterize methicillin-resistant S. aureus (MRSA) from bulk tank milk samples of cows, sheep, and goats collected from 49 dairy farms in the Czech Republic. Out of 52 samples, 15 were positive for MRSA. All MRSA isolates were identified using PCR, detection of the species-specific fragment SA442 and mecA gene. SCCmec, spa type, MLST, staphylococcal enterotoxins (SEs), and virulence genes were characterized. Three sequence types (ST) containing seven spa types were identified. Thirteen isolates belonged to ST398 (t011/ IVNT and V, t034/V, t1456/IVa, t1255/V, t2346/IVa and V). Other two isolates belonged to ST5/t3598/V (cow’s milk) and ST8/t064/V (goat’s milk). In six isolates, one or more SEs (seb, sed, seg, sei, sej) have been confirmed. All isolates were negative for the tested virulence genes except one isolate (ST398/t034/V, cow’s milk) which harboured the toxic shock syndrome toxin gene. The major clone, ST398 in different herds, among all MRSA positive animal species were confirmed. This result evidence wide geographical spread of ST398 across the regions in the Czech Republic and no host preference among the animal species.

Název v anglickém jazyce

Detection and characterization of methicillin-resistant Staphylococcus aureus isolated from milk samples of cows, sheep, and goats in the Czech Republic

Popis výsledku anglicky

This study aimed to detect and characterize methicillin-resistant S. aureus (MRSA) from bulk tank milk samples of cows, sheep, and goats collected from 49 dairy farms in the Czech Republic. Out of 52 samples, 15 were positive for MRSA. All MRSA isolates were identified using PCR, detection of the species-specific fragment SA442 and mecA gene. SCCmec, spa type, MLST, staphylococcal enterotoxins (SEs), and virulence genes were characterized. Three sequence types (ST) containing seven spa types were identified. Thirteen isolates belonged to ST398 (t011/ IVNT and V, t034/V, t1456/IVa, t1255/V, t2346/IVa and V). Other two isolates belonged to ST5/t3598/V (cow’s milk) and ST8/t064/V (goat’s milk). In six isolates, one or more SEs (seb, sed, seg, sei, sej) have been confirmed. All isolates were negative for the tested virulence genes except one isolate (ST398/t034/V, cow’s milk) which harboured the toxic shock syndrome toxin gene. The major clone, ST398 in different herds, among all MRSA positive animal species were confirmed. This result evidence wide geographical spread of ST398 across the regions in the Czech Republic and no host preference among the animal species.

Druh

O - Ostatní výsledky

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů