Detection and molecular characterization of methicillin-resistant Staphylococcus aureus isolated from bulk tank milk of cows, sheep and goats

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000010" target="_blank" >RIV/00027162:_____/19:N0000010 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16270/19:43877938

Výsledek na webu

<a href="https://www.liebertpub.com/doi/10.1089/fpd.2018.2511" target="_blank" >https://www.liebertpub.com/doi/10.1089/fpd.2018.2511</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1089/fpd.2018.2511" target="_blank" >10.1089/fpd.2018.2511</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Detection and molecular characterization of methicillin-resistant Staphylococcus aureus isolated from bulk tank milk of cows, sheep and goats

Popis výsledku v původním jazyce

This study is aimed at detecting and characterizing methicillin-resistant Staphylococcus aureus (MRSA) from bulk tank milk samples of cows, sheep and goats collected from dairy farms in the Czech Republic. Bulk tank milk samples were collected on 49 farms, 14 of which were found to be positive for MRSA. All MRSA isolates were identified by using PCR detection of the species-specific fragment SA442 and mecA gene. The presence of SCCmec, spa type, MLST, enterotoxin encoding genes (ses), virulence genes and φSa3 prophage was assessed and susceptibility to cefoxitin and tetracycline was tested using the disk diffusion method. Three sequence types (ST) containing seven spa types were identified. Twelve isolates belonged to ST398 spa types t011/SCCmec IVa (goat’s milk), t011/SCCmec V (cow’s milk), t034/SCCmec V (cow’s milk), t1456/SCCmec IVa (cow’s milk), t1255/SCCmec V (cow’s milk) and t2346/SCCmec V (cow’s and goat’s milk). Another two isolates belonged to ST5/t3598/SCCmec IVa (cow’s milk) and ST8/t064/SCCmec IVNT (goat’s milk). In six isolates, one or more ses encoding genes (seb, sed, seg, sei, sej) were confirmed. One isolate (ST398/t034/SCCmec V, cow’s milk) harboured the toxic shock toxin syndrome (tst) gene. Another two isolates (ST398/t1456/SCCmec IVa and ST5/t3598/SCCmec IVa, cow's milk) harboured the staphylokinase (sak) gene and φSa3 prophage, and the latter was the only tetracycline susceptible isolate. However, none of the isolates was positive for Panton-Valentine leucocidin (pvl) or exfoliative toxins A & B (eta&b). ST398, was confirmed to be the major clone among all MRSA positive animal species in different herds. This result provides evidence of the wide geographical spread of ST398 across different regions of the Czech Republic and no host preference among the animal species. Therefore, when evaluating the occupational and foodborne risks, MRSA carriage and infection have to be taken into account.

Název v anglickém jazyce

Detection and molecular characterization of methicillin-resistant Staphylococcus aureus isolated from bulk tank milk of cows, sheep and goats

Popis výsledku anglicky

This study is aimed at detecting and characterizing methicillin-resistant Staphylococcus aureus (MRSA) from bulk tank milk samples of cows, sheep and goats collected from dairy farms in the Czech Republic. Bulk tank milk samples were collected on 49 farms, 14 of which were found to be positive for MRSA. All MRSA isolates were identified by using PCR detection of the species-specific fragment SA442 and mecA gene. The presence of SCCmec, spa type, MLST, enterotoxin encoding genes (ses), virulence genes and φSa3 prophage was assessed and susceptibility to cefoxitin and tetracycline was tested using the disk diffusion method. Three sequence types (ST) containing seven spa types were identified. Twelve isolates belonged to ST398 spa types t011/SCCmec IVa (goat’s milk), t011/SCCmec V (cow’s milk), t034/SCCmec V (cow’s milk), t1456/SCCmec IVa (cow’s milk), t1255/SCCmec V (cow’s milk) and t2346/SCCmec V (cow’s and goat’s milk). Another two isolates belonged to ST5/t3598/SCCmec IVa (cow’s milk) and ST8/t064/SCCmec IVNT (goat’s milk). In six isolates, one or more ses encoding genes (seb, sed, seg, sei, sej) were confirmed. One isolate (ST398/t034/SCCmec V, cow’s milk) harboured the toxic shock toxin syndrome (tst) gene. Another two isolates (ST398/t1456/SCCmec IVa and ST5/t3598/SCCmec IVa, cow's milk) harboured the staphylokinase (sak) gene and φSa3 prophage, and the latter was the only tetracycline susceptible isolate. However, none of the isolates was positive for Panton-Valentine leucocidin (pvl) or exfoliative toxins A & B (eta&b). ST398, was confirmed to be the major clone among all MRSA positive animal species in different herds. This result provides evidence of the wide geographical spread of ST398 across different regions of the Czech Republic and no host preference among the animal species. Therefore, when evaluating the occupational and foodborne risks, MRSA carriage and infection have to be taken into account.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Foodborne Pathogens and Disease

ISSN

1535-3141

e-ISSN

—

Svazek periodika

16

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

68-73

Kód UT WoS článku

000451272800001

EID výsledku v databázi Scopus

2-s2.0-85060379298