MONITORING OF THE PRESENCE OF CARP EDEMA VIRUS (CEV) IN AQUACULTURE OF THE CZECH REPUBLIC IN 2017

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F18%3AN0000137" target="_blank" >RIV/00027162:_____/18:N0000137 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.was.org/Meetings/Default.aspx?code=Aqua18" target="_blank" >https://www.was.org/Meetings/Default.aspx?code=Aqua18</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

MONITORING OF THE PRESENCE OF CARP EDEMA VIRUS (CEV) IN AQUACULTURE OF THE CZECH REPUBLIC IN 2017

Popis výsledku v původním jazyce

Konference AQUA 2018, Montpellier, France, August 25-29 2018. In the last few years, increasing of spring mortality of common carp in European ponds was recorded. It affected mostly larger, almost market-sized fish. In 2011, first detection of a virus from the case of spring mortality of carp was noticed in CEFAS (Way et al., 2013). It was very similar to Carp Edema Virus (CEV; family Poxviridae) which was isolated from koi suffering from Koi Sleepy Disease in Japan in the late 1970s (Oyamatsu et al., 1997; Miyazaki et al., 2005). Since then, CEV-like virus has been considered as one of possible causal agents of SCMS (Lewisch et al., 2015). Clinical as well as pathological symptoms of the „CEV disease“ in carp noticeably remind koi herpesvirus disease (KHVD) but in case of CEV, the symptoms and mortality usually evolve in lower temperature (8 – 20 °C). In the Czech Republic, mortality caused by CEV was first confirmed by PCR in 2015 (two-round PCR developed by CEFAS was used) in archived samples from 2013 and 2014 (Vesely et al., 2015). Since that time, the diagnostic of increased mortality of carp in ponds after the winter was focused on CEV, especially when the clinical and pathological signs were as lethargy, asphyxia, gathering of fish at the surface and near the shore or inflow, irregular mucus layer on the skin, sunken eyes, necrotic gill etc. and the temperature of water ranged from 5 to 13 °C. Till the end of 2016, sixteen suspect cases of “spring carp mortality” were investigated by PCR. Seven from them were CEV DNA positive. In 2017, the national grant project supported by Ministry of Agriculture of the Czech Republic started. Twenty localities were investigated from 30th March to 28th June. Four from them were koi breeds, sixteen carp ponds. In all localities, increasing mortality was reported. Testing for CEV presence employed a two-round PCR method developed at CEFAS, with external primers yielding a product of 528 bp, and internal primers yielding a product of 478 bp (Way et al., 2014). Nine localities were CEV DNA positive (3 koi, 6 carp) but not all fish from these localities were positive although they showed similar signs. In 2017, the first case of autumn mortality of carp connected with the presence of CEV was registered. This outbreak started some days after the transfer of harvested market-sized fish to a storage pond. In this case, all investigated fish were CEV DNA positive. Mortality reached 100 % during three weeks. It is necessary to continue in the monitoring of the presence of CEV in carps suffering from typical clinical and pathological signs in spring and autumn to understand better the role of CEV in these epizootics and the pathogenesis of this disease.

Název v anglickém jazyce

MONITORING OF THE PRESENCE OF CARP EDEMA VIRUS (CEV) IN AQUACULTURE OF THE CZECH REPUBLIC IN 2017

Popis výsledku anglicky

Konference AQUA 2018, Montpellier, France, August 25-29 2018. In the last few years, increasing of spring mortality of common carp in European ponds was recorded. It affected mostly larger, almost market-sized fish. In 2011, first detection of a virus from the case of spring mortality of carp was noticed in CEFAS (Way et al., 2013). It was very similar to Carp Edema Virus (CEV; family Poxviridae) which was isolated from koi suffering from Koi Sleepy Disease in Japan in the late 1970s (Oyamatsu et al., 1997; Miyazaki et al., 2005). Since then, CEV-like virus has been considered as one of possible causal agents of SCMS (Lewisch et al., 2015). Clinical as well as pathological symptoms of the „CEV disease“ in carp noticeably remind koi herpesvirus disease (KHVD) but in case of CEV, the symptoms and mortality usually evolve in lower temperature (8 – 20 °C). In the Czech Republic, mortality caused by CEV was first confirmed by PCR in 2015 (two-round PCR developed by CEFAS was used) in archived samples from 2013 and 2014 (Vesely et al., 2015). Since that time, the diagnostic of increased mortality of carp in ponds after the winter was focused on CEV, especially when the clinical and pathological signs were as lethargy, asphyxia, gathering of fish at the surface and near the shore or inflow, irregular mucus layer on the skin, sunken eyes, necrotic gill etc. and the temperature of water ranged from 5 to 13 °C. Till the end of 2016, sixteen suspect cases of “spring carp mortality” were investigated by PCR. Seven from them were CEV DNA positive. In 2017, the national grant project supported by Ministry of Agriculture of the Czech Republic started. Twenty localities were investigated from 30th March to 28th June. Four from them were koi breeds, sixteen carp ponds. In all localities, increasing mortality was reported. Testing for CEV presence employed a two-round PCR method developed at CEFAS, with external primers yielding a product of 528 bp, and internal primers yielding a product of 478 bp (Way et al., 2014). Nine localities were CEV DNA positive (3 koi, 6 carp) but not all fish from these localities were positive although they showed similar signs. In 2017, the first case of autumn mortality of carp connected with the presence of CEV was registered. This outbreak started some days after the transfer of harvested market-sized fish to a storage pond. In this case, all investigated fish were CEV DNA positive. Mortality reached 100 % during three weeks. It is necessary to continue in the monitoring of the presence of CEV in carps suffering from typical clinical and pathological signs in spring and autumn to understand better the role of CEV in these epizootics and the pathogenesis of this disease.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/QK1710114" target="_blank" >QK1710114: Nová virová onemocnění v chovech kapra obecného - diagnostika a prevence</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů