Comparison of diagnostic methods in paratuberculosis infected herd

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F18%3AN0000242" target="_blank" >RIV/00027162:_____/18:N0000242 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.paratuberculosis.net/proceedings/proc14.pdf" target="_blank" >http://www.paratuberculosis.net/proceedings/proc14.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of diagnostic methods in paratuberculosis infected herd

Popis výsledku v původním jazyce

14th International Colloquium on Paratuberculosis, 4.-8.6.2018, Mexiko. Causative agent of paratuberculosis (PTB), Mycobacterium avium subspecies paratuberculosis (MAP) can be diagnosed by several methods. These are direct (real time PCR, cultivation) and indirect (ELISA) detection methods. Its use depends on what we expect from the method. Whether we use the cheap, fast, sensitive method or only in this time the “fashion” method. On the base of the field data, the statistics can be used to determine the reliability and comparability of the individual diagnostic methods used in common veterinary practice. The objective of this study was to compare results of three diagnostic method (ELISA, cultivation and real time PCR) applied on the naturally infected cows in different stage of the MAP infection Two media and two small breeds were selected. In all four breeds PTB occurred, including occasional clinical forms. From all animals over the age of 18 months, faeces and blood were collected. DNA from faeces was isolated by the QIAamp DNA Stool kit according to the slightly modified manufacturer's instructions. For Cultivation the faeces were decontaminated and inoculated onto special solid growth media with supplement. Cultivation was done in 37 °C for three months. Blood samples were taken from serum. Antibody diagnosis was based on the ELISA method using the ID Screen Paratuberculosis Indirect Kit. The Cultivation, Real Time PCR, and ELISA results were statistically evaluated using Fisher's test and chi-square test. And statistical model was created. Within the study, 650 animals originated from four cattle farms were tested for PTB. From all animals, 304 (46.8%), 109 (16.8%) and 76 (11.7%) were determined as positive using Real Time PCR, ELISA assay and culture, respectively. The results of all three methods start to correspond with each other in animals being in clinical phase of infection and shedding more than 104 of MAP in gram of faeces. Statistical analysis showed that the animal determined as positive in qPCR examination will be assessed as positive only in 35% using ELISA test. Using culture, the probability of positivity will be even lower (25%). Statistical analysis showed that the animal determined as positive in qPCR examination will be assessed as positive only in 35% using ELISA test. Using culture, the probability of positivity will be even lower (25%).

Název v anglickém jazyce

Comparison of diagnostic methods in paratuberculosis infected herd

Popis výsledku anglicky

14th International Colloquium on Paratuberculosis, 4.-8.6.2018, Mexiko. Causative agent of paratuberculosis (PTB), Mycobacterium avium subspecies paratuberculosis (MAP) can be diagnosed by several methods. These are direct (real time PCR, cultivation) and indirect (ELISA) detection methods. Its use depends on what we expect from the method. Whether we use the cheap, fast, sensitive method or only in this time the “fashion” method. On the base of the field data, the statistics can be used to determine the reliability and comparability of the individual diagnostic methods used in common veterinary practice. The objective of this study was to compare results of three diagnostic method (ELISA, cultivation and real time PCR) applied on the naturally infected cows in different stage of the MAP infection Two media and two small breeds were selected. In all four breeds PTB occurred, including occasional clinical forms. From all animals over the age of 18 months, faeces and blood were collected. DNA from faeces was isolated by the QIAamp DNA Stool kit according to the slightly modified manufacturer's instructions. For Cultivation the faeces were decontaminated and inoculated onto special solid growth media with supplement. Cultivation was done in 37 °C for three months. Blood samples were taken from serum. Antibody diagnosis was based on the ELISA method using the ID Screen Paratuberculosis Indirect Kit. The Cultivation, Real Time PCR, and ELISA results were statistically evaluated using Fisher's test and chi-square test. And statistical model was created. Within the study, 650 animals originated from four cattle farms were tested for PTB. From all animals, 304 (46.8%), 109 (16.8%) and 76 (11.7%) were determined as positive using Real Time PCR, ELISA assay and culture, respectively. The results of all three methods start to correspond with each other in animals being in clinical phase of infection and shedding more than 104 of MAP in gram of faeces. Statistical analysis showed that the animal determined as positive in qPCR examination will be assessed as positive only in 35% using ELISA test. Using culture, the probability of positivity will be even lower (25%). Statistical analysis showed that the animal determined as positive in qPCR examination will be assessed as positive only in 35% using ELISA test. Using culture, the probability of positivity will be even lower (25%).

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/LO1218" target="_blank" >LO1218: Zdravé zvíře jako zdroj zdravé potraviny</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů