Microbial quality of colostrum from czech dairy herds

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F18%3AN0000262" target="_blank" >RIV/00027162:_____/18:N0000262 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027014:_____/18:N0000008

Výsledek na webu

<a href="https://www.bcva.eu/" target="_blank" >https://www.bcva.eu/</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Microbial quality of colostrum from czech dairy herds

Popis výsledku v původním jazyce

Cattle Practice OCTOBER 2018 Volume 26 Part 2 BCVA Congress 2018 Additional posters, Jurys Inn Hinckley Island Hotel, Watling Street, Hinckley, LE10 3JA, England, 18.–20. october 2018 - poster. The aim of our study was quantitative and qualitative evaluation of microorganisms present in freshly milked colostrum in Czech Republic. In 38 common dairy herds (21 Czech Fleckvieh, 14 Holstein, 3 both breeds), 972 colostrum samples (531 Czech Fleckvieh, 441 Holstein cattle) were taken from 2015 to 2017. Farmers collected the samples at the first milking after calving into sterile 30 mL vessels. Subsequently, the samples were frozen and transported into the laboratory. The studied parameters were: Total plate count (TPC), coliform count (CC) and Gram negative (G-) non-coliform count (NCC). Plate count agar was used for TPC and MacConkey agar was used for coliform count CC and NCC determination in 1 mL of colostrum. Two cut-off levels recommended in previous studies were considered – the limit for TPC <100,000 cfu/mL and CC <10,000 cfu/mL according to McGuirk and Collins (2004) and the limit for TPC <20,000 cfu/mL and CC <100 cfu/mL according to Heinrichs and Jones (2017). The limit for NCC <5,000 cfu/mL was used, as recommended in both studies. Out of total 972 samples, in 108 samples from 18 farms (6 samples each) the species of microorganism were determined using MALDI-TOF method. Findings were classified into 4 groups: faecal contaminants, environmental contaminants, skin and mucosal microorganisms, and Gram positive (G+) mammary pathogens. The data was evaluated in Statistica CZ 10.0.Only 27.1% samples met the requirement of TPC <100,000 cfu/mL (median 490,000 cfu/mL) and the stricter limit <20,000 met 12.2% of samples. However 88.6% and 38.6% of samples were below the limits for CC (<10,000 and <100 cfu/mL), median 220 cfu/mL. And 84.5% of samples met the limit for NCC (<5,000 cfu/mL), median 110 cfu/mL. TPC was significantly lower (p<0.05) in the Holstein herds (median 270,000) compared to 750,000 cfu/mL in Czech Fleckvieh herds (Man-Whitney U-test). The proportions of samples containing faecal contaminants, environmental contaminants, and skin and mucosal microorganisms were comparatively high with 79.2%, 72.9%, and 80.4%, respectively. The most frequently isolated representatives of the above groups were Enterococcus spp. (in 71.0% of samples), Moraxella osloensis (42.1% samples) and Staphylococcus spp. (41.1% samples). G+ mastitis agents were detected in 20.6% of samples, Streptococcus parauberis in 10.3%, Streptococcus uberis in 6.5% and Staphylococcus aureus in 5.6% of samples. We can conclude that bacterial contamination of Czech colostrum is very high. Basing our data on species composition, the decisive part of bacterial contamination within the most of colostrum samples were the most likely caused by poor hygiene of housing and by an insufficient preparation of the udder during the first milking after parturition, which is normally done outside the main milking parlour. Poor sanitation of the special milking equipment can also be key factor.

Název v anglickém jazyce

Microbial quality of colostrum from czech dairy herds

Popis výsledku anglicky

Cattle Practice OCTOBER 2018 Volume 26 Part 2 BCVA Congress 2018 Additional posters, Jurys Inn Hinckley Island Hotel, Watling Street, Hinckley, LE10 3JA, England, 18.–20. october 2018 - poster. The aim of our study was quantitative and qualitative evaluation of microorganisms present in freshly milked colostrum in Czech Republic. In 38 common dairy herds (21 Czech Fleckvieh, 14 Holstein, 3 both breeds), 972 colostrum samples (531 Czech Fleckvieh, 441 Holstein cattle) were taken from 2015 to 2017. Farmers collected the samples at the first milking after calving into sterile 30 mL vessels. Subsequently, the samples were frozen and transported into the laboratory. The studied parameters were: Total plate count (TPC), coliform count (CC) and Gram negative (G-) non-coliform count (NCC). Plate count agar was used for TPC and MacConkey agar was used for coliform count CC and NCC determination in 1 mL of colostrum. Two cut-off levels recommended in previous studies were considered – the limit for TPC <100,000 cfu/mL and CC <10,000 cfu/mL according to McGuirk and Collins (2004) and the limit for TPC <20,000 cfu/mL and CC <100 cfu/mL according to Heinrichs and Jones (2017). The limit for NCC <5,000 cfu/mL was used, as recommended in both studies. Out of total 972 samples, in 108 samples from 18 farms (6 samples each) the species of microorganism were determined using MALDI-TOF method. Findings were classified into 4 groups: faecal contaminants, environmental contaminants, skin and mucosal microorganisms, and Gram positive (G+) mammary pathogens. The data was evaluated in Statistica CZ 10.0.Only 27.1% samples met the requirement of TPC <100,000 cfu/mL (median 490,000 cfu/mL) and the stricter limit <20,000 met 12.2% of samples. However 88.6% and 38.6% of samples were below the limits for CC (<10,000 and <100 cfu/mL), median 220 cfu/mL. And 84.5% of samples met the limit for NCC (<5,000 cfu/mL), median 110 cfu/mL. TPC was significantly lower (p<0.05) in the Holstein herds (median 270,000) compared to 750,000 cfu/mL in Czech Fleckvieh herds (Man-Whitney U-test). The proportions of samples containing faecal contaminants, environmental contaminants, and skin and mucosal microorganisms were comparatively high with 79.2%, 72.9%, and 80.4%, respectively. The most frequently isolated representatives of the above groups were Enterococcus spp. (in 71.0% of samples), Moraxella osloensis (42.1% samples) and Staphylococcus spp. (41.1% samples). G+ mastitis agents were detected in 20.6% of samples, Streptococcus parauberis in 10.3%, Streptococcus uberis in 6.5% and Staphylococcus aureus in 5.6% of samples. We can conclude that bacterial contamination of Czech colostrum is very high. Basing our data on species composition, the decisive part of bacterial contamination within the most of colostrum samples were the most likely caused by poor hygiene of housing and by an insufficient preparation of the udder during the first milking after parturition, which is normally done outside the main milking parlour. Poor sanitation of the special milking equipment can also be key factor.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/QJ1510219" target="_blank" >QJ1510219: Komplexní řízení mlezivové výživy telat a její zlepšování jako přirozený nástroj k podpoře zdraví telat, tlumení nákaz a snížení potřeby antibiotik</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů