The quality of DNA isolated from processed food and feed via different extraction procedures
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000015" target="_blank" >RIV/00027162:_____/19:N0000015 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/62157124:16270/19:43877352
Výsledek na webu
<a href="https://www.mdpi.com/1420-3049/24/6/1188" target="_blank" >https://www.mdpi.com/1420-3049/24/6/1188</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/molecules24061188" target="_blank" >10.3390/molecules24061188</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
The quality of DNA isolated from processed food and feed via different extraction procedures
Popis výsledku v původním jazyce
The extraction of DNA is a critical step for species identification by PCR analysis in processed food and feed products. In this study, eight DNA extraction procedures were compared: DNeasy Blood & Tissue Kit, DNeasy mericon Food Kit, chemagic DNA Tissue 10 Kit, Food DNA Isolation Kit, UltraPrep Genomic DNA Food Mini Prep Kit, High Pure PCR Template Preparation Kit, Phenol-chloroform extraction and NucleoSpin Food using self-prepared samples from both raw and heat-processed and/or mechanically treated muscles and different types of meat products and pet food (pork, beef and chicken). The yield, purity and suitability of DNA for PCR amplification was evaluated. Comparisons between the effectiveness of various kits were made with regard to price, labour- and time-intensiveness. It was found that the DNeasy mericon Food Kit was the optimal choice for the extraction of DNA from raw muscle, heat-treated muscle and home-made meat products from multiple and single species.
Název v anglickém jazyce
The quality of DNA isolated from processed food and feed via different extraction procedures
Popis výsledku anglicky
The extraction of DNA is a critical step for species identification by PCR analysis in processed food and feed products. In this study, eight DNA extraction procedures were compared: DNeasy Blood & Tissue Kit, DNeasy mericon Food Kit, chemagic DNA Tissue 10 Kit, Food DNA Isolation Kit, UltraPrep Genomic DNA Food Mini Prep Kit, High Pure PCR Template Preparation Kit, Phenol-chloroform extraction and NucleoSpin Food using self-prepared samples from both raw and heat-processed and/or mechanically treated muscles and different types of meat products and pet food (pork, beef and chicken). The yield, purity and suitability of DNA for PCR amplification was evaluated. Comparisons between the effectiveness of various kits were made with regard to price, labour- and time-intensiveness. It was found that the DNeasy mericon Food Kit was the optimal choice for the extraction of DNA from raw muscle, heat-treated muscle and home-made meat products from multiple and single species.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
40401 - Agricultural biotechnology and food biotechnology
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2019
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Molecules
ISSN
1420-3049
e-ISSN
—
Svazek periodika
24
Číslo periodika v rámci svazku
6
Stát vydavatele periodika
CH - Švýcarská konfederace
Počet stran výsledku
10
Strana od-do
1188
Kód UT WoS článku
000465503800150
EID výsledku v databázi Scopus
2-s2.0-85063505887