The Quality of DNA Isolated from Processed Food and Feed via Different Extraction Procedures

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F19%3A43877352" target="_blank" >RIV/62157124:16270/19:43877352 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027162:_____/19:N0000015

Výsledek na webu

<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6471455/" target="_blank" >https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6471455/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/molecules24061188" target="_blank" >10.3390/molecules24061188</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The Quality of DNA Isolated from Processed Food and Feed via Different Extraction Procedures

Popis výsledku v původním jazyce

The extraction of DNA is a critical step for species identification by PCR analysis in processed food and feed products. In this study, eight DNA extraction procedures were compared - DNeasy Blood and Tissue Kit, DNeasy mericon Food Kit, chemagic DNA Tissue 10 Kit, Food DNA Isolation Kit, UltraPrep Genomic DNA Food Mini Prep Kit, High Pure PCR Template Preparation Kit, phenolchloroform extraction, and NucleoSpin Food - using self-prepared samples from both raw and heat-processed and/or mechanically treated muscles and different types of meat products and pet food (pork, beef, and chicken). The yield, purity, and suitability of DNA for PCR amplification was evaluated. Additionally, comparisons between the effectiveness of various extraction methods were made with regard to price, and labor- and time-intensiveness. It was found that the DNeasy mericon Food Kit was the optimal choice for the extraction of DNA from raw muscle, heat-treated muscle, and homemade meat products from multiple and single species.

Název v anglickém jazyce

The Quality of DNA Isolated from Processed Food and Feed via Different Extraction Procedures

Popis výsledku anglicky

The extraction of DNA is a critical step for species identification by PCR analysis in processed food and feed products. In this study, eight DNA extraction procedures were compared - DNeasy Blood and Tissue Kit, DNeasy mericon Food Kit, chemagic DNA Tissue 10 Kit, Food DNA Isolation Kit, UltraPrep Genomic DNA Food Mini Prep Kit, High Pure PCR Template Preparation Kit, phenolchloroform extraction, and NucleoSpin Food - using self-prepared samples from both raw and heat-processed and/or mechanically treated muscles and different types of meat products and pet food (pork, beef, and chicken). The yield, purity, and suitability of DNA for PCR amplification was evaluated. Additionally, comparisons between the effectiveness of various extraction methods were made with regard to price, and labor- and time-intensiveness. It was found that the DNeasy mericon Food Kit was the optimal choice for the extraction of DNA from raw muscle, heat-treated muscle, and homemade meat products from multiple and single species.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/QJ1530107" target="_blank" >QJ1530107: Metody pro identifikaci, sledovatelnost a ověřování autenticity potravin a krmiv s komponenty živočišného původu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecules

ISSN

1420-3049

e-ISSN

—

Svazek periodika

24

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

10

Strana od-do

—

Kód UT WoS článku

000465503800150

EID výsledku v databázi Scopus

2-s2.0-85063505887