Employment of MOL-PCR in bacterial pathogen detection in small ruminants

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000108" target="_blank" >RIV/00027162:_____/19:N0000108 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Employment of MOL-PCR in bacterial pathogen detection in small ruminants

Popis výsledku v původním jazyce

xMAP CONNECT, 2019 5th ANNIVERSARY, AMSTERDAM, 5. – 6.11. 2019. In contrast to the detection of pathogens in the human diagnostic, the spectrum of available methods in the veterinary diagnostic is very low. The major consideration for farmers is the price for the test and therefore, multiplexing can decrease expenses. The aim of the study was to prepare a multiplex panel for the detection of Mycobacterium avium subspecies paratuberculosis, Corynebacterium pseudotuberculosis, Mycobacterium bovis and Mycobacterium tuberculosis complex members. These bacterial pathogens play an important role in health of small ruminants with overlap to human health. These selected zoonotic pathogens can be transmitted via colostrum, milk or fecal samples to other animals or can enter to the human food chain. The diagnostic panel intended for small ruminants was based on the MOL-PCR method followed by MagPix analysis. The validation of the multiplex assay was performed on artificially and naturally contaminated milk samples. The validated MOL-PCR assay proved to be suitable for the routine detection and identification of the above mentioned pathogens. This assay will contribute to the quick identification of infected animals and their effective removal from herd.

Název v anglickém jazyce

Employment of MOL-PCR in bacterial pathogen detection in small ruminants

Popis výsledku anglicky

xMAP CONNECT, 2019 5th ANNIVERSARY, AMSTERDAM, 5. – 6.11. 2019. In contrast to the detection of pathogens in the human diagnostic, the spectrum of available methods in the veterinary diagnostic is very low. The major consideration for farmers is the price for the test and therefore, multiplexing can decrease expenses. The aim of the study was to prepare a multiplex panel for the detection of Mycobacterium avium subspecies paratuberculosis, Corynebacterium pseudotuberculosis, Mycobacterium bovis and Mycobacterium tuberculosis complex members. These bacterial pathogens play an important role in health of small ruminants with overlap to human health. These selected zoonotic pathogens can be transmitted via colostrum, milk or fecal samples to other animals or can enter to the human food chain. The diagnostic panel intended for small ruminants was based on the MOL-PCR method followed by MagPix analysis. The validation of the multiplex assay was performed on artificially and naturally contaminated milk samples. The validated MOL-PCR assay proved to be suitable for the routine detection and identification of the above mentioned pathogens. This assay will contribute to the quick identification of infected animals and their effective removal from herd.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30303 - Infectious Diseases

Projekt

<a href="/cs/project/QK1810212" target="_blank" >QK1810212: Rychlé, komplexní a multiplexní metody pro simultánní detekci původců alimentárních onemocnění v potravinách živočišného a rostlinného původu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů