Successful detection of multiple rotavirus species from raw water intake in the wastewater treatment plant

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000181" target="_blank" >RIV/00027162:_____/19:N0000181 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Successful detection of multiple rotavirus species from raw water intake in the wastewater treatment plant

Popis výsledku v původním jazyce

6th EERVAC + 8th Rotavirus Molecular Biology Meeting, Riga, 23.-26.4.2019 - poster. The monitoring of viruses present in sewage from a wastewater treatment plant (WTP) has been described as an appropriate model to understand the spread of enteric viruses in the population served by the WTP, as influents may contain viruses shed by patients with sporadic or asymptomatic cases. In order to cover the rotavirus population, raw sewage (influent) samples were collected from two urban wastewater treatment plants during rotavirus peak season (November-December and March) in 2017-2018. Samples were filtered and then concentrated 200x (Vivaspin, Sartorius) and extracted RNA was sent for NGS to a sequencing service laboratory (SEQme, Dobříš, Czech Republic). The library was prepared with Ion Total RNA-seq Kit v2 (Thermo Fisher Scientific) and sequencing was carried out with Ion PI™ Hi-Q™ Sequencing 200 Kit, Ion PI™ Chip Kit v3. Sequencing data were processed using Torrent Suite software. Obtained reads were aligned to the available sequences of RVA, RVB and RVC. 1-4,7 million reads was obtained and around 12% of reads were mapped. So far, four influent samples from WTP were characterized. This method showed high sensitivity and proved suitable for nonconventional samples, in this case wastewater analysis. Multiple RVA genotypes as well as other rotavirus groups (RVB, RVC) were successfully detected. Even if non-A rotaviruses were not found in clinical samples in the Czech Republic yet, their detection in WTP samples proves circulation of human RVB and RVC in the population.

Název v anglickém jazyce

Successful detection of multiple rotavirus species from raw water intake in the wastewater treatment plant

Popis výsledku anglicky

6th EERVAC + 8th Rotavirus Molecular Biology Meeting, Riga, 23.-26.4.2019 - poster. The monitoring of viruses present in sewage from a wastewater treatment plant (WTP) has been described as an appropriate model to understand the spread of enteric viruses in the population served by the WTP, as influents may contain viruses shed by patients with sporadic or asymptomatic cases. In order to cover the rotavirus population, raw sewage (influent) samples were collected from two urban wastewater treatment plants during rotavirus peak season (November-December and March) in 2017-2018. Samples were filtered and then concentrated 200x (Vivaspin, Sartorius) and extracted RNA was sent for NGS to a sequencing service laboratory (SEQme, Dobříš, Czech Republic). The library was prepared with Ion Total RNA-seq Kit v2 (Thermo Fisher Scientific) and sequencing was carried out with Ion PI™ Hi-Q™ Sequencing 200 Kit, Ion PI™ Chip Kit v3. Sequencing data were processed using Torrent Suite software. Obtained reads were aligned to the available sequences of RVA, RVB and RVC. 1-4,7 million reads was obtained and around 12% of reads were mapped. So far, four influent samples from WTP were characterized. This method showed high sensitivity and proved suitable for nonconventional samples, in this case wastewater analysis. Multiple RVA genotypes as well as other rotavirus groups (RVB, RVC) were successfully detected. Even if non-A rotaviruses were not found in clinical samples in the Czech Republic yet, their detection in WTP samples proves circulation of human RVB and RVC in the population.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

20602 - Medical laboratory technology (including laboratory samples analysis; diagnostic technologies) (Biomaterials to be 2.9 [physical characteristics of living material as related to medical implants, devices, sensors])

Projekt

<a href="/cs/project/NV16-29937A" target="_blank" >NV16-29937A: Komplexní analýza humánních rotavirových infekcí v České republice včetně atypických a emergentních kmenů směřující k vývoji nových detekčních metod</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů