Is Rotavirus A the only rotavirus present in Czech human patients?
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000190" target="_blank" >RIV/00027162:_____/19:N0000190 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Is Rotavirus A the only rotavirus present in Czech human patients?
Popis výsledku v původním jazyce
22nd ESCV, Kodaň, 11. - 14. 9. 2019 - poster. The objective of our study was to elucidate whether apart from Rotavirus A (RVA) we could detect also other rotavirus species in human stool samples and waste water. We examined the set of 1500 stool samples of patients with mainly gastrointestinal disorders with the use of sensitive RT-qPCR methods (Primerdesign™ Genesig® Kit for RVA, RVB and RVC detection was compared with in-house RT-qPCR assay). The samples were collected during 2016-2018 in five microbiological diagnostic laboratories. Apart from that we examined eight samples of waste water from the intake of two big urban waste-water treatment plants with the use of next-generation sequencing (NGS). From the results of NGS of waste-water samples we may conclude that RVC and RVB are present in the Czech Republic. However, the epidemiologic relevance of those rotaviruses is probably very low or the viral loads in clinical samples is below the limit of detection.
Název v anglickém jazyce
Is Rotavirus A the only rotavirus present in Czech human patients?
Popis výsledku anglicky
22nd ESCV, Kodaň, 11. - 14. 9. 2019 - poster. The objective of our study was to elucidate whether apart from Rotavirus A (RVA) we could detect also other rotavirus species in human stool samples and waste water. We examined the set of 1500 stool samples of patients with mainly gastrointestinal disorders with the use of sensitive RT-qPCR methods (Primerdesign™ Genesig® Kit for RVA, RVB and RVC detection was compared with in-house RT-qPCR assay). The samples were collected during 2016-2018 in five microbiological diagnostic laboratories. Apart from that we examined eight samples of waste water from the intake of two big urban waste-water treatment plants with the use of next-generation sequencing (NGS). From the results of NGS of waste-water samples we may conclude that RVC and RVB are present in the Czech Republic. However, the epidemiologic relevance of those rotaviruses is probably very low or the viral loads in clinical samples is below the limit of detection.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
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OECD FORD obor
30302 - Epidemiology
Návaznosti výsledku
Projekt
<a href="/cs/project/NV16-29937A" target="_blank" >NV16-29937A: Komplexní analýza humánních rotavirových infekcí v České republice včetně atypických a emergentních kmenů směřující k vývoji nových detekčních metod</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2019
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů