Genotype distribution of rotavirus A infection in the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000182" target="_blank" >RIV/00027162:_____/19:N0000182 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Genotype distribution of rotavirus A infection in the Czech Republic

Popis výsledku v původním jazyce

6th EERVAC + 8th Rotavirus Molecular Biology Meeting, Riga, 23.-26.4.2019 - poster. Phylogenetic analyses of rotavirus genome reveal repeated intersections between the evolution of human and animal rotavirus strains. The interspecies transmission and subsequent gene reassortment are important mechanisms driving the diversity of rotaviruses and enabling the emergence of new pathogenic strains. Between 2016 and 2018 a total of 1,608 faecal specimens were collected from patients of all age groups and were tested for RVA by an in-house RT-qPCR. Out of these, 309 (19.2%) were positive. So far, G- and P-typing was successfully carried out in 122 human RVA strains either with the help of Sanger or next-generation sequencing. RVA typing of the Czech strains determined five VP7 genotypes – G1 (41%), G9 (30%), G8 (18%), G3 (6%) and G2 (5%). Nearly all VP4 genotypes were described as P[8], except in five strains where genotype combinations G2P[4] (3x), G9P[4] (1x) and G3P[9] (1x) were found. In this study, the uncommon feline-like rotavirus strain G3P[9] was detected in a 20-month-old female child. Another reassortant strain G3P[8] with an equine-like VP7 segment was isolated from multiple cases of GIT infection since 2017. The constant surveillance of rotavirus genotypes with the use of sequencing methods is of high importance as commonly used genotyping with RT-PCR cannot identify some less common or novel reassortant RVA strains.

Název v anglickém jazyce

Genotype distribution of rotavirus A infection in the Czech Republic

Popis výsledku anglicky

6th EERVAC + 8th Rotavirus Molecular Biology Meeting, Riga, 23.-26.4.2019 - poster. Phylogenetic analyses of rotavirus genome reveal repeated intersections between the evolution of human and animal rotavirus strains. The interspecies transmission and subsequent gene reassortment are important mechanisms driving the diversity of rotaviruses and enabling the emergence of new pathogenic strains. Between 2016 and 2018 a total of 1,608 faecal specimens were collected from patients of all age groups and were tested for RVA by an in-house RT-qPCR. Out of these, 309 (19.2%) were positive. So far, G- and P-typing was successfully carried out in 122 human RVA strains either with the help of Sanger or next-generation sequencing. RVA typing of the Czech strains determined five VP7 genotypes – G1 (41%), G9 (30%), G8 (18%), G3 (6%) and G2 (5%). Nearly all VP4 genotypes were described as P[8], except in five strains where genotype combinations G2P[4] (3x), G9P[4] (1x) and G3P[9] (1x) were found. In this study, the uncommon feline-like rotavirus strain G3P[9] was detected in a 20-month-old female child. Another reassortant strain G3P[8] with an equine-like VP7 segment was isolated from multiple cases of GIT infection since 2017. The constant surveillance of rotavirus genotypes with the use of sequencing methods is of high importance as commonly used genotyping with RT-PCR cannot identify some less common or novel reassortant RVA strains.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30302 - Epidemiology

Projekt

<a href="/cs/project/NV16-29937A" target="_blank" >NV16-29937A: Komplexní analýza humánních rotavirových infekcí v České republice včetně atypických a emergentních kmenů směřující k vývoji nových detekčních metod</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů