Emergence of rare bovine-human reassortant DS-1-like rotavirus A strains with G8P[8] genotype in human patients in the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000191" target="_blank" >RIV/00027162:_____/19:N0000191 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00159816:_____/19:00072457 RIV/00216224:14110/19:00108575

Výsledek na webu

<a href="https://www.mdpi.com/1999-4915/11/11/1015" target="_blank" >https://www.mdpi.com/1999-4915/11/11/1015</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/v11111015" target="_blank" >10.3390/v11111015</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Emergence of rare bovine-human reassortant DS-1-like rotavirus A strains with G8P[8] genotype in human patients in the Czech Republic

Popis výsledku v původním jazyce

Group A Rotaviruses (RVA) are the leading cause of acute gastroenteritis in children and a major cause of childhood mortality in low-income countries. RVAs are mostly host-specific, however, interspecies transmission and reassortment between human and animal RVAs contribute significantly to their genetic diversity. We investigated VP7 and VP4 genotypes of RVA isolated from 225 stool specimens collected from Czech patients with gastroenteritis during 2016-2019. The most abundant genotypes were G1P[8] (42.7%), G3P[8] (11.1%), G9P[8] (9.8%), G2P[4] (4.4%), G4P[8] (1.3%), G12P[8] (1.3%) and surprisingly G8P[8] (9.3%). Sequence analysis of G8P[8] revealed the highest nucleotide similarity of all Czech G8 sequences to the G8P[8] rotavirus strains isolated in Vietnam in 2014/2015. The whole genome backbone of the Czech G8 strains was determined with the use of next-generation sequencing as DS-1-like. The phylogenetic analysis of all segments clustered the Czech isolates with RVA strains formerly described in Southeast Asia which had emerged following genetic reassortment between bovine and human RVAs. This is the first time that bovine-human DS 1-like G8P[8] strains are detected at high rate in human patients in Central Europe. Whether the emergence of this unusual genotype reflects establishing of a new RVA strain in the population will require continuous monitoring of rotavirus epidemiology.

Název v anglickém jazyce

Emergence of rare bovine-human reassortant DS-1-like rotavirus A strains with G8P[8] genotype in human patients in the Czech Republic

Popis výsledku anglicky

Group A Rotaviruses (RVA) are the leading cause of acute gastroenteritis in children and a major cause of childhood mortality in low-income countries. RVAs are mostly host-specific, however, interspecies transmission and reassortment between human and animal RVAs contribute significantly to their genetic diversity. We investigated VP7 and VP4 genotypes of RVA isolated from 225 stool specimens collected from Czech patients with gastroenteritis during 2016-2019. The most abundant genotypes were G1P[8] (42.7%), G3P[8] (11.1%), G9P[8] (9.8%), G2P[4] (4.4%), G4P[8] (1.3%), G12P[8] (1.3%) and surprisingly G8P[8] (9.3%). Sequence analysis of G8P[8] revealed the highest nucleotide similarity of all Czech G8 sequences to the G8P[8] rotavirus strains isolated in Vietnam in 2014/2015. The whole genome backbone of the Czech G8 strains was determined with the use of next-generation sequencing as DS-1-like. The phylogenetic analysis of all segments clustered the Czech isolates with RVA strains formerly described in Southeast Asia which had emerged following genetic reassortment between bovine and human RVAs. This is the first time that bovine-human DS 1-like G8P[8] strains are detected at high rate in human patients in Central Europe. Whether the emergence of this unusual genotype reflects establishing of a new RVA strain in the population will require continuous monitoring of rotavirus epidemiology.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10607 - Virology

Projekt

<a href="/cs/project/NV16-29937A" target="_blank" >NV16-29937A: Komplexní analýza humánních rotavirových infekcí v České republice včetně atypických a emergentních kmenů směřující k vývoji nových detekčních metod</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Viruses

ISSN

1999-4915

e-ISSN

—

Svazek periodika

11

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

14

Strana od-do

1015

Kód UT WoS článku

000502292300043

EID výsledku v databázi Scopus

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