Molecular diversity of Listeria monocytogenes isolated from human invasive listeriosis in the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000186" target="_blank" >RIV/00027162:_____/19:N0000186 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.eccmidlive.org/" target="_blank" >https://www.eccmidlive.org/</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Molecular diversity of Listeria monocytogenes isolated from human invasive listeriosis in the Czech Republic

Popis výsledku v původním jazyce

29th ECCMID, Amsterdam, Netherlands, 13.–16. 4. 2019 – poster. Considering the high mortality of listeriosis, the global food trade, changes in dietary habits and demographic changes leading to the increase of sensitive groups of population, it is important to monitor the occurrence of this disease and to perform detail characteristics of L. monocytogenes (LM). This study was aimed at the evaluation of the diversity and possible epidemic relationships of human LM obtained in the Czech Republic (CR) between 2013 and 2018. LM isolates (149) from clinical cases reported from 2013 to 2018 (July) were obtained from regional laboratories in the Czech Republic. All isolates were characterized by serotyping, macrorestriction analysis and whole genome sequencing using Illumina platform. Strains of LM ST8 (serotype 1/2a) and ST6 (4b) predominated in the human population. High incidence of LM ST8 was influenced by the listeriosis outbreak in the Moravian-Silesian Region associated with the consumption ready-to-eat turkey products. In the monitored period, four other LM clusters with possible epidemic link based on cgMLST were detected. Two clusters (five and four cases) belonged to ST6. Frequent occurrence of LM ST6 may be associated with a potentially higher virulence of CC6. In contrast with isolates of ST8, majority (57 %) of LM ST6 were associated with CNS infections or neonatal listeriosis. Another two clusters included isolates of serotype 1/2a belonging to ST1350 (5 cases) and ST7 (5 cases). Stress survival islet 1 (SSI-1), which contributes to the growth of LM in suboptimal conditions, was identified in the genome of isolates ST8 involved in the aforementioned outbreak and also in isolates of ST1350 and ST7. The cgMLST allowed an unambiguous differentiation of isolates involved in listeriosis cases and their inclusion to clusters with a possible epidemic link. The occurrence of specific STs in human cases may be either related to their higher virulence or to the presence of genes responsible for better adaptation of LM in food chain leading to their overexposure of the community.

Název v anglickém jazyce

Molecular diversity of Listeria monocytogenes isolated from human invasive listeriosis in the Czech Republic

Popis výsledku anglicky

29th ECCMID, Amsterdam, Netherlands, 13.–16. 4. 2019 – poster. Considering the high mortality of listeriosis, the global food trade, changes in dietary habits and demographic changes leading to the increase of sensitive groups of population, it is important to monitor the occurrence of this disease and to perform detail characteristics of L. monocytogenes (LM). This study was aimed at the evaluation of the diversity and possible epidemic relationships of human LM obtained in the Czech Republic (CR) between 2013 and 2018. LM isolates (149) from clinical cases reported from 2013 to 2018 (July) were obtained from regional laboratories in the Czech Republic. All isolates were characterized by serotyping, macrorestriction analysis and whole genome sequencing using Illumina platform. Strains of LM ST8 (serotype 1/2a) and ST6 (4b) predominated in the human population. High incidence of LM ST8 was influenced by the listeriosis outbreak in the Moravian-Silesian Region associated with the consumption ready-to-eat turkey products. In the monitored period, four other LM clusters with possible epidemic link based on cgMLST were detected. Two clusters (five and four cases) belonged to ST6. Frequent occurrence of LM ST6 may be associated with a potentially higher virulence of CC6. In contrast with isolates of ST8, majority (57 %) of LM ST6 were associated with CNS infections or neonatal listeriosis. Another two clusters included isolates of serotype 1/2a belonging to ST1350 (5 cases) and ST7 (5 cases). Stress survival islet 1 (SSI-1), which contributes to the growth of LM in suboptimal conditions, was identified in the genome of isolates ST8 involved in the aforementioned outbreak and also in isolates of ST1350 and ST7. The cgMLST allowed an unambiguous differentiation of isolates involved in listeriosis cases and their inclusion to clusters with a possible epidemic link. The occurrence of specific STs in human cases may be either related to their higher virulence or to the presence of genes responsible for better adaptation of LM in food chain leading to their overexposure of the community.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/NV16-31488A" target="_blank" >NV16-31488A: Molekulární epidemiologie listerióz s využitím metod sekvenace nové generace</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů