Genetic variety of plasmid mediated colistin resistance in Gram-negative bacteria from raw meat products

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000187" target="_blank" >RIV/00027162:_____/19:N0000187 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.anses.fr/fr/content/protection-de-la-sant%C3%A9-publique-impact-du-s%C3%A9quen%C3%A7age-complet-des-g%C3%A9nomes-des-pathog%C3%A8nes-d" target="_blank" >https://www.anses.fr/fr/content/protection-de-la-sant%C3%A9-publique-impact-du-s%C3%A9quen%C3%A7age-complet-des-g%C3%A9nomes-des-pathog%C3%A8nes-d</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Genetic variety of plasmid mediated colistin resistance in Gram-negative bacteria from raw meat products

Popis výsledku v původním jazyce

Foodborne pathogens and whole genome sequencing: impact on public health protection, Paris, France, 26.-28. 3. 2019 – poster. This study was focused on plasmid characteristics of Enterobacteriaceae isolates with mcr-1 gene and one Aeromonas spp. isolate carrying both mcr-3 and mcr-7-like genes obtained from retailed raw meat products. Altogether, 17 colistin resistant isolates carrying mcr genes originated from 12 meat samples (turkey, rabbit and quail meat). The sequencing was performed using Illumina MiSeq and Oxford Nanopore MinION platform. The hybrid assembly of the genomes showed three plasmid types (IncX4, IncHI2 and IncI2) carrying the mcr-1 gene in tested isolates. The most prevalent plasmid type carrying mcr-1 was IncX4 detected in E. coli (n=8) and K. pneumoniae (n=2) isolated from meat products of different origin (Czech Republic, Poland, Germany, Brazil). Four E. coli isolates from turkey meat of Polish origin had localized mcr-1 on plasmid type IncHI2 which varied in their size (between ~153 kb to 284 kb) and carried other resistance genes. The isolates of E. coli and C. braakii from one rabbit meat sample from China carried mcr-1 gene on IncI2 plasmid (~60 kb). The results of this study indicate a possible spread of plasmid mediated colistin resistance via raw meat market between bacteria of different genus. IncX4 plasmids carrying mcr-1 gene were found to be conserved among species of Enterobacteriaceae isolated from raw meat products of different origin. While the most of tested IncX4 and IncI2 plasmids had similar size and carried only mcr-1, the tested IncHI2 plasmids carrying mcr-1 gene were diverse in size and in the content of resistance genes. The presence of mcr-3 and mcr-7-like genes on plasmids in colistin resistant Aeromonas spp. isolate was not confirmed.

Název v anglickém jazyce

Genetic variety of plasmid mediated colistin resistance in Gram-negative bacteria from raw meat products

Popis výsledku anglicky

Foodborne pathogens and whole genome sequencing: impact on public health protection, Paris, France, 26.-28. 3. 2019 – poster. This study was focused on plasmid characteristics of Enterobacteriaceae isolates with mcr-1 gene and one Aeromonas spp. isolate carrying both mcr-3 and mcr-7-like genes obtained from retailed raw meat products. Altogether, 17 colistin resistant isolates carrying mcr genes originated from 12 meat samples (turkey, rabbit and quail meat). The sequencing was performed using Illumina MiSeq and Oxford Nanopore MinION platform. The hybrid assembly of the genomes showed three plasmid types (IncX4, IncHI2 and IncI2) carrying the mcr-1 gene in tested isolates. The most prevalent plasmid type carrying mcr-1 was IncX4 detected in E. coli (n=8) and K. pneumoniae (n=2) isolated from meat products of different origin (Czech Republic, Poland, Germany, Brazil). Four E. coli isolates from turkey meat of Polish origin had localized mcr-1 on plasmid type IncHI2 which varied in their size (between ~153 kb to 284 kb) and carried other resistance genes. The isolates of E. coli and C. braakii from one rabbit meat sample from China carried mcr-1 gene on IncI2 plasmid (~60 kb). The results of this study indicate a possible spread of plasmid mediated colistin resistance via raw meat market between bacteria of different genus. IncX4 plasmids carrying mcr-1 gene were found to be conserved among species of Enterobacteriaceae isolated from raw meat products of different origin. While the most of tested IncX4 and IncI2 plasmids had similar size and carried only mcr-1, the tested IncHI2 plasmids carrying mcr-1 gene were diverse in size and in the content of resistance genes. The presence of mcr-3 and mcr-7-like genes on plasmids in colistin resistant Aeromonas spp. isolate was not confirmed.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/NV18-09-00254" target="_blank" >NV18-09-00254: Sentinelová studie výskytu na plazmid vázané (přenositelné) rezistence ke kolistinu u Enterobacteriaceae v České republice</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů