Dissemination and comparison of genetic determinants of mcr-mediated colistin resistance in Enterobacteriaceae via retailed raw meat products

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000307" target="_blank" >RIV/00027162:_____/19:N0000307 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/19:00111721 RIV/62157124:16270/19:43878261 RIV/62157124:16810/19:43878261

Výsledek na webu

<a href="https://www.frontiersin.org/articles/10.3389/fmicb.2019.02824/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fmicb.2019.02824/full</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Dissemination and comparison of genetic determinants of mcr-mediated colistin resistance in Enterobacteriaceae via retailed raw meat products

Popis výsledku v původním jazyce

The global food chain may significantly support the dissemination of bacteria resistant to antibiotics around the world. This study was aimed at determining the prevalence and genetic characteristics of Enterobacteriaceae, with mcr-mediated colistin resistance, from retail meat of different origins. Bacteria of Enterobacteriaceae carrying the mcr-1 gene were detected in 21% (18/86) of the examined samples, especially in turkey meat and liver originating from the EU and non-EU countries (19%) and in rabbit meat imported from China (2%). The examined samples of chicken and other poultry meat and liver, pork and beef were negative for the presence of bacteria carrying mcr-1 to mcr-5 genes. A huge number of isolates belonging to E. coli (n=54), K. pneumoniae (n=6) and Citrobacter braakii (n=1) carrying the mcr-1 gene were obtained. Despite the high heterogeneity of the tested isolates, the mcr-1 gene was localized on only three types of plasmids (IncX4, IncHI2, IncI2). The most frequent type of plasmid was IncX4, which carried the mcr-1 gene in 77% of E. coli and K. pneumoniae isolates from turkey meat and liver samples from the Czech Republic, Germany, Poland and Brazil. Our findings indicate a highly probable interspecies transfer of IncX4 and IncI2 plasmids within one sample meat. The co-resistance of plasmid-mediated colistin resistance encoded by the mcr-1 and ESBL genes was detected in 18% of the isolates. Another noteworthy finding was the fosA3 gene coding for fosfomycin resistance in a multi-drug resistant isolate of E. coli from rabbit meat imported from China. The observed high level of Enterobacteriaceae with plasmids carrying mcr-1 gene from retail meat reflects the need for a Europe-wide monitoring of mcr-mediated colistin resistance through the whole food chain.

Název v anglickém jazyce

Dissemination and comparison of genetic determinants of mcr-mediated colistin resistance in Enterobacteriaceae via retailed raw meat products

Popis výsledku anglicky

The global food chain may significantly support the dissemination of bacteria resistant to antibiotics around the world. This study was aimed at determining the prevalence and genetic characteristics of Enterobacteriaceae, with mcr-mediated colistin resistance, from retail meat of different origins. Bacteria of Enterobacteriaceae carrying the mcr-1 gene were detected in 21% (18/86) of the examined samples, especially in turkey meat and liver originating from the EU and non-EU countries (19%) and in rabbit meat imported from China (2%). The examined samples of chicken and other poultry meat and liver, pork and beef were negative for the presence of bacteria carrying mcr-1 to mcr-5 genes. A huge number of isolates belonging to E. coli (n=54), K. pneumoniae (n=6) and Citrobacter braakii (n=1) carrying the mcr-1 gene were obtained. Despite the high heterogeneity of the tested isolates, the mcr-1 gene was localized on only three types of plasmids (IncX4, IncHI2, IncI2). The most frequent type of plasmid was IncX4, which carried the mcr-1 gene in 77% of E. coli and K. pneumoniae isolates from turkey meat and liver samples from the Czech Republic, Germany, Poland and Brazil. Our findings indicate a highly probable interspecies transfer of IncX4 and IncI2 plasmids within one sample meat. The co-resistance of plasmid-mediated colistin resistance encoded by the mcr-1 and ESBL genes was detected in 18% of the isolates. Another noteworthy finding was the fosA3 gene coding for fosfomycin resistance in a multi-drug resistant isolate of E. coli from rabbit meat imported from China. The observed high level of Enterobacteriaceae with plasmids carrying mcr-1 gene from retail meat reflects the need for a Europe-wide monitoring of mcr-mediated colistin resistance through the whole food chain.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Frontiers in Microbiology

ISSN

1664-302X

e-ISSN

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Svazek periodika

10

Číslo periodika v rámci svazku

DEC 2019

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

10

Strana od-do

2824

Kód UT WoS článku

000504719200001

EID výsledku v databázi Scopus

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