First Report of plasmid mediated colistin resistance from wildlife in Russia

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F19%3A43878237" target="_blank" >RIV/62157124:16270/19:43878237 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16810/19:43878237

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

First Report of plasmid mediated colistin resistance from wildlife in Russia

Popis výsledku v původním jazyce

The aim of this study was to select Enterobacteriaceae isolates with plasmid-mediated colistin resistance genes from cloacal swabs of Eurasian Black and Red Kites. Isolates (13.8%, n=168) that showed reduced susceptibility to colistin (3.5mg/L) were identified using MALDI-TOF MS and tested for mobile colistin resistance encoded by mcr genes using PCR. One mcr-1-positive E. coli isolate was detected and selected for further testing. The isolate was resistant to colistin (MIC &gt; 4mg/L) and susceptible to 18 antibiotics by broth dilution and disk diffusion methods, respectively. WGS using MiSeq (Illumina) revealed the mcr-1-positive E. coli sample belonged to ST2280. The mcr-1 gene was located on conjugative IncI2 plasmid pDR164 (59891 bp, GenBank number MK542639). Plasmid pDR164 showed similarity to other mcr-1-carrying IncI2 plasmids found in E. coli isolates from swine, poultry, human and sewage in USA, Vietnam, Argentina and China. Comparison of pDR164 with other plasmids revealed high similarity in the plasmid backbone and the region carrying mcr. In this study, we report the first mcr-1-positive isolate in wildlife in Russia

Název v anglickém jazyce

First Report of plasmid mediated colistin resistance from wildlife in Russia

Popis výsledku anglicky

The aim of this study was to select Enterobacteriaceae isolates with plasmid-mediated colistin resistance genes from cloacal swabs of Eurasian Black and Red Kites. Isolates (13.8%, n=168) that showed reduced susceptibility to colistin (3.5mg/L) were identified using MALDI-TOF MS and tested for mobile colistin resistance encoded by mcr genes using PCR. One mcr-1-positive E. coli isolate was detected and selected for further testing. The isolate was resistant to colistin (MIC &gt; 4mg/L) and susceptible to 18 antibiotics by broth dilution and disk diffusion methods, respectively. WGS using MiSeq (Illumina) revealed the mcr-1-positive E. coli sample belonged to ST2280. The mcr-1 gene was located on conjugative IncI2 plasmid pDR164 (59891 bp, GenBank number MK542639). Plasmid pDR164 showed similarity to other mcr-1-carrying IncI2 plasmids found in E. coli isolates from swine, poultry, human and sewage in USA, Vietnam, Argentina and China. Comparison of pDR164 with other plasmids revealed high similarity in the plasmid backbone and the region carrying mcr. In this study, we report the first mcr-1-positive isolate in wildlife in Russia

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/NV18-09-00605" target="_blank" >NV18-09-00605: Enterobacteriaceae rezistentní ke karbapenemům a kolistinu: koncept jednoho zdraví v hodnocení humánních a jiných zdrojů rizikových mechanizmů antimikrobiální rezistence</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů