The effect of oral administration of microplastics with an emphasis on rainbow trout intestinal microbiota

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F20%3AN0000097" target="_blank" >RIV/00027162:_____/20:N0000097 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The effect of oral administration of microplastics with an emphasis on rainbow trout intestinal microbiota

Popis výsledku v původním jazyce

The production of plastics is increasing dramatically throughout the world and is considered to be a serious threat to the aquatic environment. Microplastics are particles with a size between 20 µm – 5 mm, able to permeate the intestinal endothelium and reach the tissues. Our study is focused on polystyrene microparticles and their impact on rainbow trout (Oncorhynchus mykiss) organism. The experiment will be performed in accordance with the Fish Juvenile Growth Test (OECD 215) and the microparticles will be incorporated into commercial pellets for experimental fish. The fish will be fed twice a day 1.5% of their wet weight. In total, three experimental groups will be tested – microplastics at concentrations of 0.5%, 2% and 5% in comparison with the control group without addition of microplastics. Each tested group will consist of 20 fish and the experiment will be performed in duplicate. The whole experiment will be carried out during 8 weeks with a 14-day period of acclimatization and 6 weeks of the experimental phase. At the end of the experimental phase, the effect on hematological, biochemical, histological and immunological indices in a time and dose-dependent manner will be evaluated. The main task of our study will be analysis of intestinal microbiota with an emphasis on Lactobacillus spp. and Brevinema mutual ratio after microplastics exposure where the DNA will be isolated and submitted to qRT-PCR.

Název v anglickém jazyce

The effect of oral administration of microplastics with an emphasis on rainbow trout intestinal microbiota

Popis výsledku anglicky

The production of plastics is increasing dramatically throughout the world and is considered to be a serious threat to the aquatic environment. Microplastics are particles with a size between 20 µm – 5 mm, able to permeate the intestinal endothelium and reach the tissues. Our study is focused on polystyrene microparticles and their impact on rainbow trout (Oncorhynchus mykiss) organism. The experiment will be performed in accordance with the Fish Juvenile Growth Test (OECD 215) and the microparticles will be incorporated into commercial pellets for experimental fish. The fish will be fed twice a day 1.5% of their wet weight. In total, three experimental groups will be tested – microplastics at concentrations of 0.5%, 2% and 5% in comparison with the control group without addition of microplastics. Each tested group will consist of 20 fish and the experiment will be performed in duplicate. The whole experiment will be carried out during 8 weeks with a 14-day period of acclimatization and 6 weeks of the experimental phase. At the end of the experimental phase, the effect on hematological, biochemical, histological and immunological indices in a time and dose-dependent manner will be evaluated. The main task of our study will be analysis of intestinal microbiota with an emphasis on Lactobacillus spp. and Brevinema mutual ratio after microplastics exposure where the DNA will be isolated and submitted to qRT-PCR.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/EF16_019%2F0000869" target="_blank" >EF16_019/0000869: Udržitelná produkce zdravých ryb v různých akvakulturních systémech - PROFISH</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů