The effect of oral microplastics application with emphasis on rainbow trout intestinal microflora

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F22%3AN0000057" target="_blank" >RIV/00027162:_____/22:N0000057 - isvavai.cz</a>

Výsledek na webu

<a href="https://icbf-congress.com/en/" target="_blank" >https://icbf-congress.com/en/</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The effect of oral microplastics application with emphasis on rainbow trout intestinal microflora

Popis výsledku v původním jazyce

The production of plastics is increasing dramatically throughout the world and is considered to be serious threat for the aquatic environment. Microplastics are particles with a size between 20 µm – 5 mm, are able to permeate intestinal endothelium and reach the tissues. Our study is focused on the polystyrene microparticles and their impact on rainbow trout (Oncorhynchus mykiss) organism. The experiment will be performed in accordance to the Fish Juvenile Growth Test (OECD 215) while the microparticles will be incorporated into the commercial pellets of experimental fish. The fish will be fed twice a day with amount 2% of their wet weight, three experimental groups will be tested – microplastics in concentration 0.5%, 2% and 5%, control group without addition of microplastics. One group will be consisted from 20 fish pieces and experiment will be performed in duplicate. Whole experiment will be in duration 8 weeks with 14-days long period of acclimatization and 6 weeks of experimental phase. At the end of experimental phase, the effect on hematological, biochemical, histological and immunological indices in time and dosedepent manner will be evaluated. Main task of our study will be analysis of intestinal microflora with emphasis on Lactobacillus spp. and Brevinema mutual ratio after microplastics exposure when the DNA will be isolated and submitted for qRT-PCR. Project was funded by the ERDF/ESF “PROFISH” [no. CZ.02.1.01/0.0/0.0/16_019/0000869].

Název v anglickém jazyce

The effect of oral microplastics application with emphasis on rainbow trout intestinal microflora

Popis výsledku anglicky

The production of plastics is increasing dramatically throughout the world and is considered to be serious threat for the aquatic environment. Microplastics are particles with a size between 20 µm – 5 mm, are able to permeate intestinal endothelium and reach the tissues. Our study is focused on the polystyrene microparticles and their impact on rainbow trout (Oncorhynchus mykiss) organism. The experiment will be performed in accordance to the Fish Juvenile Growth Test (OECD 215) while the microparticles will be incorporated into the commercial pellets of experimental fish. The fish will be fed twice a day with amount 2% of their wet weight, three experimental groups will be tested – microplastics in concentration 0.5%, 2% and 5%, control group without addition of microplastics. One group will be consisted from 20 fish pieces and experiment will be performed in duplicate. Whole experiment will be in duration 8 weeks with 14-days long period of acclimatization and 6 weeks of experimental phase. At the end of experimental phase, the effect on hematological, biochemical, histological and immunological indices in time and dosedepent manner will be evaluated. Main task of our study will be analysis of intestinal microflora with emphasis on Lactobacillus spp. and Brevinema mutual ratio after microplastics exposure when the DNA will be isolated and submitted for qRT-PCR. Project was funded by the ERDF/ESF “PROFISH” [no. CZ.02.1.01/0.0/0.0/16_019/0000869].

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/EF16_019%2F0000869" target="_blank" >EF16_019/0000869: Udržitelná produkce zdravých ryb v různých akvakulturních systémech - PROFISH</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů