A multicenter proposal for a fast tool to screen biosecure chicken flocks for the foodborne pathogen campylobacter

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F20%3AN0000111" target="_blank" >RIV/00027162:_____/20:N0000111 - isvavai.cz</a>

Výsledek na webu

<a href="https://aem.asm.org/content/86/20/e01051-20" target="_blank" >https://aem.asm.org/content/86/20/e01051-20</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1128/AEM.01051-20" target="_blank" >10.1128/AEM.01051-20</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A multicenter proposal for a fast tool to screen biosecure chicken flocks for the foodborne pathogen campylobacter

Popis výsledku v původním jazyce

The present multi-centre study aimed at assessing the performance of air sampling as a novel method for monitoring Campylobacter in biosecured poultry farms. We compared, using a harmonized procedure, the bacteriological isolation protocol (ISO 10272-1:2017), and a single real-time PCR method on air filter samples. Air samples and boot swabs were collected from 62 biosecured flocks from five European countries during the summer of 2019. For air filters, the frequency of PCR-positive findings was statistically significantly higher (n=36; 58%) compared to the cultivation methods (p<0.01; Standardized Residuals). The cultivation protocols (one with Bolton enrichment and one with Preston enrichment) were comparable to each other but returned fewer positive samples (0%-8%). The association between type of sample and frequency of PCR-positive findings was confirmed (p<0.01; Fisher´s exact test), although no culture-positive air filters were detected using direct plating. For the boot swabs, the highest number of positive samples were detected after enrichment in Preston broth (n=23; 37%), followed by direct plating after homogenization in Preston (n=21; 34%) or Bolton (n=20; 32%). In conclusion, air sampling combined with real time PCR is proposed as a multi-purpose, low-cost and convenient screening method that can be up to 4 times faster and 4 times more sensitive than the current boot-swab testing scheme used for screening biosecured broiler production. It is important to note that the Norwegian flocks, a country known to be free of Campylobacter in biosecured chicken production, still appeared Campylobacter-free in the new sensitive method.

Název v anglickém jazyce

A multicenter proposal for a fast tool to screen biosecure chicken flocks for the foodborne pathogen campylobacter

Popis výsledku anglicky

The present multi-centre study aimed at assessing the performance of air sampling as a novel method for monitoring Campylobacter in biosecured poultry farms. We compared, using a harmonized procedure, the bacteriological isolation protocol (ISO 10272-1:2017), and a single real-time PCR method on air filter samples. Air samples and boot swabs were collected from 62 biosecured flocks from five European countries during the summer of 2019. For air filters, the frequency of PCR-positive findings was statistically significantly higher (n=36; 58%) compared to the cultivation methods (p<0.01; Standardized Residuals). The cultivation protocols (one with Bolton enrichment and one with Preston enrichment) were comparable to each other but returned fewer positive samples (0%-8%). The association between type of sample and frequency of PCR-positive findings was confirmed (p<0.01; Fisher´s exact test), although no culture-positive air filters were detected using direct plating. For the boot swabs, the highest number of positive samples were detected after enrichment in Preston broth (n=23; 37%), followed by direct plating after homogenization in Preston (n=21; 34%) or Bolton (n=20; 32%). In conclusion, air sampling combined with real time PCR is proposed as a multi-purpose, low-cost and convenient screening method that can be up to 4 times faster and 4 times more sensitive than the current boot-swab testing scheme used for screening biosecured broiler production. It is important to note that the Norwegian flocks, a country known to be free of Campylobacter in biosecured chicken production, still appeared Campylobacter-free in the new sensitive method.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/ED2.1.00%2F19.0385" target="_blank" >ED2.1.00/19.0385: Rozvoj výzkumných kapacit Centra AdmireVet</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Applied and environmental microbiology

ISSN

0099-2240

e-ISSN

1098-5336

Svazek periodika

86

Číslo periodika v rámci svazku

20

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

"e01051-20"

Kód UT WoS článku

000582928300006

EID výsledku v databázi Scopus

2-s2.0-85092682418