Soil protein as a potential antimicrobial agent against methicillin–resistant Staphylococcus aureus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F20%3AN0000129" target="_blank" >RIV/00027162:_____/20:N0000129 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/86652079:_____/20:00525401 RIV/62156489:43210/20:43918043 RIV/00216305:26620/20:PU137242

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0013935120302139?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0013935120302139?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.envres.2020.109320" target="_blank" >10.1016/j.envres.2020.109320</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Soil protein as a potential antimicrobial agent against methicillin–resistant Staphylococcus aureus

Popis výsledku v původním jazyce

Recently, the interest is increasing to find alternatives to replace the usage of antibiotics since their massive and improper usage enhance the antibiotic resistance in human pathogens. In this study, for the first time we showed that the soil proteins have very high antibacterial activity (98% of growth inhibition) against methicillin resistant Staphylococcus aureus (MRSA), one of the most threatening human pathogens. We found that the protein extract (C3) from the forest with past intensive management showed higher antibacterial activity than that of unmanaged forest. The MIC and IC50 were found to be 30 and 15.0 μg protein g−1 dry soil respectively. C3 was found to kill the bacteria by cell wall disruption and genotoxicity which was confirmed by optical and fluorescent microscopy and comet assay. According to qPCR study, the mecA (the antibiotic resistant gene) expression in MRSA was found to be down-regulated after C3 treatment. In contrast, C3 showed no hemolytic toxicity on human red blood cells which was confirmed by hemolytic assay. According to ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS), 144 proteins were identified in C3 among which the majority belonged to Gram negative bacteria (45.8%). Altogether, our results will help to develop novel, costeffective, non-toxic and highly efficient antibacterial medicines from natural sources against antibiotic resistant infections.

Název v anglickém jazyce

Soil protein as a potential antimicrobial agent against methicillin–resistant Staphylococcus aureus

Popis výsledku anglicky

Recently, the interest is increasing to find alternatives to replace the usage of antibiotics since their massive and improper usage enhance the antibiotic resistance in human pathogens. In this study, for the first time we showed that the soil proteins have very high antibacterial activity (98% of growth inhibition) against methicillin resistant Staphylococcus aureus (MRSA), one of the most threatening human pathogens. We found that the protein extract (C3) from the forest with past intensive management showed higher antibacterial activity than that of unmanaged forest. The MIC and IC50 were found to be 30 and 15.0 μg protein g−1 dry soil respectively. C3 was found to kill the bacteria by cell wall disruption and genotoxicity which was confirmed by optical and fluorescent microscopy and comet assay. According to qPCR study, the mecA (the antibiotic resistant gene) expression in MRSA was found to be down-regulated after C3 treatment. In contrast, C3 showed no hemolytic toxicity on human red blood cells which was confirmed by hemolytic assay. According to ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS), 144 proteins were identified in C3 among which the majority belonged to Gram negative bacteria (45.8%). Altogether, our results will help to develop novel, costeffective, non-toxic and highly efficient antibacterial medicines from natural sources against antibiotic resistant infections.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ENVIRONMENTAL RESEARCH

ISSN

0013-9351

e-ISSN

1096-0953

Svazek periodika

188

Číslo periodika v rámci svazku

SEP 2020

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

13

Strana od-do

"109320"

Kód UT WoS článku

000564662200021

EID výsledku v databázi Scopus

2-s2.0-85086369098