Vliv probiotik na imunitní odpověď pstruha duhového; XXII. Konference mladých vědeckých pracovníků s mezinárodní účastí Brno, 20.5. 2020
Popis výsledku
Probiotics are widely used to stimulate the fish immune response and increase resistance to diseases. In this study, the effect of continual and cyclic feed supplementation of probiotics on the immune response was investigated. Head kidney samples were taken, and the lymphocyte transformation test was performed. This method was also applied to the samples taken after the subsequent experimental challenge with Aeromonas salmonicida, and to the samples taken after vaccination with a commercial vaccine against Yersinia ruckeri. In the latter, in addition to non-specific stimulation, the cells were also specifically stimulated with the antigen isolated from the vaccine. Plasma levels of specific antibodies were also measured. With both specific and non-specific stimulation, the proliferative response increased with time. No statistically significant effect of probiotic supplementation on lymphocyte proliferation was observed, the groups fed with probiotics showed only slightly higher response to specific stimulation after vaccination compared to the control. However, a significant increase in levels of specific antibodies was observed in the probiotic-fed groups after vaccination.
Klíčová slova
LactobacillusOncorhynchus mykissAeromonas salmonicidavaccineproliferation
Identifikátory výsledku
Kód výsledku v IS VaVaI
Výsledek na webu
https://fvhe.vfu.cz/files/upload/media-verejnost/sborniky-z-konferenci/KMVP%202020_sborn%C3%ADk.pdf
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
čeština
Název v původním jazyce
Vliv probiotik na imunitní odpověď pstruha duhového; XXII. Konference mladých vědeckých pracovníků s mezinárodní účastí Brno, 20.5. 2020
Popis výsledku v původním jazyce
Probiotics are widely used to stimulate the fish immune response and increase resistance to diseases. In this study, the effect of continual and cyclic feed supplementation of probiotics on the immune response was investigated. Head kidney samples were taken, and the lymphocyte transformation test was performed. This method was also applied to the samples taken after the subsequent experimental challenge with Aeromonas salmonicida, and to the samples taken after vaccination with a commercial vaccine against Yersinia ruckeri. In the latter, in addition to non-specific stimulation, the cells were also specifically stimulated with the antigen isolated from the vaccine. Plasma levels of specific antibodies were also measured. With both specific and non-specific stimulation, the proliferative response increased with time. No statistically significant effect of probiotic supplementation on lymphocyte proliferation was observed, the groups fed with probiotics showed only slightly higher response to specific stimulation after vaccination compared to the control. However, a significant increase in levels of specific antibodies was observed in the probiotic-fed groups after vaccination.
Název v anglickém jazyce
Influence of probiotics on the immune response in rainbow trout
Popis výsledku anglicky
Probiotics are widely used to stimulate the fish immune response and increase resistance to diseases. In this study, the effect of continual and cyclic feed supplementation of probiotics on the immune response was investigated. Head kidney samples were taken, and the lymphocyte transformation test was performed. This method was also applied to the samples taken after the subsequent experimental challenge with Aeromonas salmonicida, and to the samples taken after vaccination with a commercial vaccine against Yersinia ruckeri. In the latter, in addition to non-specific stimulation, the cells were also specifically stimulated with the antigen isolated from the vaccine. Plasma levels of specific antibodies were also measured. With both specific and non-specific stimulation, the proliferative response increased with time. No statistically significant effect of probiotic supplementation on lymphocyte proliferation was observed, the groups fed with probiotics showed only slightly higher response to specific stimulation after vaccination compared to the control. However, a significant increase in levels of specific antibodies was observed in the probiotic-fed groups after vaccination.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
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OECD FORD obor
40301 - Veterinary science
Návaznosti výsledku
Projekt
EF16_019/0000869: Udržitelná produkce zdravých ryb v různých akvakulturních systémech - PROFISH
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2020
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Základní informace
Druh výsledku
O - Ostatní výsledky
OECD FORD
Veterinary science
Rok uplatnění
2020