Effect of dietary fumonisin on the immune response in Rainbow Trout (Oncorhynchus mykiss)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000188" target="_blank" >RIV/00027162:_____/21:N0000188 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.delegate-reg.co.uk/eafp-2021/" target="_blank" >https://www.delegate-reg.co.uk/eafp-2021/</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effect of dietary fumonisin on the immune response in Rainbow Trout (Oncorhynchus mykiss)

Popis výsledku v původním jazyce

The effects of mycotoxins have been investigated in different animal species; however, more information about their impact on the fish immune response is still required. Fumonisins, produced by several Fusarium species, represent the most common mycotoxins in plant meals. They can cause major health problems in fish, including both immunostimulation and immunosuppression, and their influence needs to be examined, especially in the main aquaculture species. Methodology: Fumonisins were administered to rainbow trout (Oncorhynchus mykiss) in feed for a period of 10 weeks. At week 6, some of the fish from the fumonisin group and from the control group were vaccinated against Yersinia ruckeri. Samples of the head kidney were taken after euthanasia of the fish at weeks 3 and 10, and non-specific mitogen-driven as well as specific antigen-driven lymphocyte proliferation assay was performed. At weeks 9 and 10, specific antibodies were also determined by an in-house ELISA. Results: With non-specific stimulation, the fish from both fumonisin groups showed a significant increase in proliferative activity after vaccination, indicating a pro-inflammatory immune reaction. The results were similar in the non-vaccinated control. Significantly lower proliferation levels were observed in the vaccinated compared to the non-vaccinated fish from the control group. With specific stimulation, significantly higher values were detected in the vaccinated fish from the fumonisin group compared to the vaccinated control. At week 9, levels of specific antibodies were significantly increased in the vaccinated fumonisin group compared to the non-vaccinated fish. However, at week 10, the control fish showed similar results with even higher values. The enhanced immune reaction, which occurred very quickly in the vaccinated fish from the fumonisin group, may be indicative of pro-inflammatory changes. Conclusions: A significant effect of fumonisins on the immune response of rainbow trout was observed. Alterations in lymphocyte proliferative activity and in the production of specific antibodies confirm the negative impact of these toxins on fish health.

Název v anglickém jazyce

Effect of dietary fumonisin on the immune response in Rainbow Trout (Oncorhynchus mykiss)

Popis výsledku anglicky

The effects of mycotoxins have been investigated in different animal species; however, more information about their impact on the fish immune response is still required. Fumonisins, produced by several Fusarium species, represent the most common mycotoxins in plant meals. They can cause major health problems in fish, including both immunostimulation and immunosuppression, and their influence needs to be examined, especially in the main aquaculture species. Methodology: Fumonisins were administered to rainbow trout (Oncorhynchus mykiss) in feed for a period of 10 weeks. At week 6, some of the fish from the fumonisin group and from the control group were vaccinated against Yersinia ruckeri. Samples of the head kidney were taken after euthanasia of the fish at weeks 3 and 10, and non-specific mitogen-driven as well as specific antigen-driven lymphocyte proliferation assay was performed. At weeks 9 and 10, specific antibodies were also determined by an in-house ELISA. Results: With non-specific stimulation, the fish from both fumonisin groups showed a significant increase in proliferative activity after vaccination, indicating a pro-inflammatory immune reaction. The results were similar in the non-vaccinated control. Significantly lower proliferation levels were observed in the vaccinated compared to the non-vaccinated fish from the control group. With specific stimulation, significantly higher values were detected in the vaccinated fish from the fumonisin group compared to the vaccinated control. At week 9, levels of specific antibodies were significantly increased in the vaccinated fumonisin group compared to the non-vaccinated fish. However, at week 10, the control fish showed similar results with even higher values. The enhanced immune reaction, which occurred very quickly in the vaccinated fish from the fumonisin group, may be indicative of pro-inflammatory changes. Conclusions: A significant effect of fumonisins on the immune response of rainbow trout was observed. Alterations in lymphocyte proliferative activity and in the production of specific antibodies confirm the negative impact of these toxins on fish health.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/EF16_019%2F0000869" target="_blank" >EF16_019/0000869: Udržitelná produkce zdravých ryb v různých akvakulturních systémech - PROFISH</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů