Dietary fumonisins affect the immune response of rainbow trout (Oncorhynchus mykiss)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000189" target="_blank" >RIV/00027162:_____/21:N0000189 - isvavai.cz</a>

Výsledek na webu

<a href="https://eposters.blob.core.windows.net/eas-eposters/AE2021AbstractBook.pdf" target="_blank" >https://eposters.blob.core.windows.net/eas-eposters/AE2021AbstractBook.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Dietary fumonisins affect the immune response of rainbow trout (Oncorhynchus mykiss)

Popis výsledku v původním jazyce

"Introduction: Mycotoxins and their effects have been examined in different animal species; however, more information about their impact on the fish immune response is still required. Fumonisins, produced by several Fusarium species, represent the most common mycotoxins in plant meals, which have been more frequently used in fish feed production in recent years. These toxins can cause major health problems in fish (Oliveira & Vasconcelos 2020), including both immunostimulation and immunosuppression (Riley et al. 1996; Pestka et al. 2004). Their effects need to be investigated more, especially in the main aquaculture species. Materials and Methods: Rainbow trout (Oncorhynchus mykiss) kept in recirculating systems were fed fumonisins for a period of 10 weeks. Some of the fish from the fumonisin group and from the control group were vaccinated against Yersinia ruckeri at week 6. At weeks 3 and 10, samples of the head kidney were taken after euthanasia of the fish (6–10 fish from each group). Non-specific mitogen-driven (100 µg/ml phytohaemagglutinin) as well as specific antigen-driven lymphocyte proliferation assay was performed, with leukocytes isolated by density gradient centrifugation. The test was evaluated by an ELISA-based assay using bromodeoxyuridine. Levels of specific antibodies were also determined by an in-house ELISA at weeks 9 and 10. Results: Non-specific stimulation showed a significant increase in proliferative activity after vaccination against Y. ruckeri in the fish from both fumonisin groups (vaccinated and non-vaccinated), indicating a pro-inflammatory immune reaction. Similar results were obtained in the non-vaccinated control. The vaccinated compared to the non-vaccinated fish from the control group showed significantly lower proliferation levels. With specific stimulation, significantly higher values were detected in the vaccinated fish from the fumonisin group compared to the vaccinated control. Levels of specific antibodies were significantly increased in the vaccinated fumonisin group compared to the non-vaccinated fish at week 9. However, at week 10, the control fish showed similar results with even higher values. The enhanced immune reaction, which occurred very quickly in the vaccinated fish from the fumonisin group, may be indicative of pro-inflammatory changes (Ellis 1999). Conclusions: Dietary fumonisins caused an activation of the immune system of rainbow trout after vaccination against Y. ruckeri, indicating a pro-inflammatory immune reaction. Alterations in lymphocyte proliferative activity and in the production of specific antibodies confirm a negative impact of these toxins on the fish immune response."

Název v anglickém jazyce

Dietary fumonisins affect the immune response of rainbow trout (Oncorhynchus mykiss)

Popis výsledku anglicky

"Introduction: Mycotoxins and their effects have been examined in different animal species; however, more information about their impact on the fish immune response is still required. Fumonisins, produced by several Fusarium species, represent the most common mycotoxins in plant meals, which have been more frequently used in fish feed production in recent years. These toxins can cause major health problems in fish (Oliveira & Vasconcelos 2020), including both immunostimulation and immunosuppression (Riley et al. 1996; Pestka et al. 2004). Their effects need to be investigated more, especially in the main aquaculture species. Materials and Methods: Rainbow trout (Oncorhynchus mykiss) kept in recirculating systems were fed fumonisins for a period of 10 weeks. Some of the fish from the fumonisin group and from the control group were vaccinated against Yersinia ruckeri at week 6. At weeks 3 and 10, samples of the head kidney were taken after euthanasia of the fish (6–10 fish from each group). Non-specific mitogen-driven (100 µg/ml phytohaemagglutinin) as well as specific antigen-driven lymphocyte proliferation assay was performed, with leukocytes isolated by density gradient centrifugation. The test was evaluated by an ELISA-based assay using bromodeoxyuridine. Levels of specific antibodies were also determined by an in-house ELISA at weeks 9 and 10. Results: Non-specific stimulation showed a significant increase in proliferative activity after vaccination against Y. ruckeri in the fish from both fumonisin groups (vaccinated and non-vaccinated), indicating a pro-inflammatory immune reaction. Similar results were obtained in the non-vaccinated control. The vaccinated compared to the non-vaccinated fish from the control group showed significantly lower proliferation levels. With specific stimulation, significantly higher values were detected in the vaccinated fish from the fumonisin group compared to the vaccinated control. Levels of specific antibodies were significantly increased in the vaccinated fumonisin group compared to the non-vaccinated fish at week 9. However, at week 10, the control fish showed similar results with even higher values. The enhanced immune reaction, which occurred very quickly in the vaccinated fish from the fumonisin group, may be indicative of pro-inflammatory changes (Ellis 1999). Conclusions: Dietary fumonisins caused an activation of the immune system of rainbow trout after vaccination against Y. ruckeri, indicating a pro-inflammatory immune reaction. Alterations in lymphocyte proliferative activity and in the production of specific antibodies confirm a negative impact of these toxins on the fish immune response."

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/EF16_019%2F0000869" target="_blank" >EF16_019/0000869: Udržitelná produkce zdravých ryb v různých akvakulturních systémech - PROFISH</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů