Detection of Mycobacterium avium subspecies paratuberculosis in environmental samples from infected Czech dairy herds

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000002" target="_blank" >RIV/00027162:_____/21:N0000002 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.agriculturejournals.cz/publicFiles/168_2020-VETMED.pdf" target="_blank" >https://www.agriculturejournals.cz/publicFiles/168_2020-VETMED.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17221/168/2020-VETMED" target="_blank" >10.17221/168/2020-VETMED</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Detection of Mycobacterium avium subspecies paratuberculosis in environmental samples from infected Czech dairy herds

Popis výsledku v původním jazyce

The objective of the present study was to evaluate suitability of environmental sampling for screening Czech dairy herds to detect Mycobacterium avium ssp. paratuberculosis (MAP) and to find the most convenient location for MAP detection in the lactating cow area. Environmental samples (ES, n = 72) from milking parlour holding pen (n = 19), milking alleyways (n = 19) and free-stall alleyways (n = 34) from 19 herds were simultaneously tested to detect MAP by quantitative PCR (qPCR) and bacterial culture. Eight and 13 samples from milking parlour holding pens, 12 and 11 samples from milking alleyways and 11 and 18 samples from free-stall alleyways were qPCR and culture positive, respectively. The 4.6 times higher probability to be culture than qPCR positive was detected for assessable MAP detection results from free-stall alleyways (P-value 0.0086, OR 4.5728) and no association was found between the results from milking parlour holding pens (P-value 0.1914) and milking alleyways (P-value >0.9999) and the diagnostic method used. The percentage of qPCR-positive samples in the tested locations was detected for milking alleyways (63.2%), free-stall alleyways and milking parlour holding pen. The herd infectious status was in agreement with 16 (84.2%), 14 (73.7%) and 12 (63.2%) qPCR results from milking alleyways, free-stall alleyways and milking parlour holding pen, respectively. No statistically significant differences were detected for these results (P-value 0.3961). MAP was detected by qPCR and culture in all three locations where ES were collected. We suggested environmental sampling followed by MAP detection by qPCR as an easy-to-do time-saving protocol for MAP screening in Czech dairy herds. Although the milking alleyways seem to be the most convenient location for environmental sampling, this assumption was not statistically supported.

Název v anglickém jazyce

Detection of Mycobacterium avium subspecies paratuberculosis in environmental samples from infected Czech dairy herds

Popis výsledku anglicky

The objective of the present study was to evaluate suitability of environmental sampling for screening Czech dairy herds to detect Mycobacterium avium ssp. paratuberculosis (MAP) and to find the most convenient location for MAP detection in the lactating cow area. Environmental samples (ES, n = 72) from milking parlour holding pen (n = 19), milking alleyways (n = 19) and free-stall alleyways (n = 34) from 19 herds were simultaneously tested to detect MAP by quantitative PCR (qPCR) and bacterial culture. Eight and 13 samples from milking parlour holding pens, 12 and 11 samples from milking alleyways and 11 and 18 samples from free-stall alleyways were qPCR and culture positive, respectively. The 4.6 times higher probability to be culture than qPCR positive was detected for assessable MAP detection results from free-stall alleyways (P-value 0.0086, OR 4.5728) and no association was found between the results from milking parlour holding pens (P-value 0.1914) and milking alleyways (P-value >0.9999) and the diagnostic method used. The percentage of qPCR-positive samples in the tested locations was detected for milking alleyways (63.2%), free-stall alleyways and milking parlour holding pen. The herd infectious status was in agreement with 16 (84.2%), 14 (73.7%) and 12 (63.2%) qPCR results from milking alleyways, free-stall alleyways and milking parlour holding pen, respectively. No statistically significant differences were detected for these results (P-value 0.3961). MAP was detected by qPCR and culture in all three locations where ES were collected. We suggested environmental sampling followed by MAP detection by qPCR as an easy-to-do time-saving protocol for MAP screening in Czech dairy herds. Although the milking alleyways seem to be the most convenient location for environmental sampling, this assumption was not statistically supported.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/QK1820086" target="_blank" >QK1820086: Paratuberkulóza – výskyt a certifikační program v chovech skotu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinární medicína

ISSN

0375-8427

e-ISSN

1805-9392

Svazek periodika

66

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

1-7

Kód UT WoS článku

000606544600001

EID výsledku v databázi Scopus

2-s2.0-85099110543