MOL-PCR and xMAP Technology: A Multiplex System for Fast Detection of Food- and Waterborne Viruses

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000078" target="_blank" >RIV/00027162:_____/21:N0000078 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60162694:G32__/21:N0000003 RIV/00216224:14310/21:00122387 RIV/62157124:16270/21:43879156

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S1525157821000866?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1525157821000866?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jmoldx.2021.03.005" target="_blank" >10.1016/j.jmoldx.2021.03.005</a>

Jazyk výsledku

angličtina

Název v původním jazyce

MOL-PCR and xMAP Technology: A Multiplex System for Fast Detection of Food- and Waterborne Viruses

Popis výsledku v původním jazyce

Viruses are common causes of food- and water-borne diseases worldwide. Conventional identification of these pathogenic agents is based on cultivation, antigen detection, electron microscopy or standardized real-time polymerase chain reaction systems. Recent technological advancements in sample processing and detection methods are currently more and more focused on fast and robust analysis. Following this approach in this study, we utilized a rapid multiplexing technology, which can detect a broad spectrum of pathogenic viruses connected to food or water contamination. We have designed a new semi-quantitative magnetic bead - based multiplex panel for simultaneous detection of several targets in one reaction. The panel includes adenoviruses 40/41 (AdV), rotavirus A (RVA), norovirus (NoV), hepatitis E virus (HEV), hepatitis A virus (HAV) and target for external control of the system. To evaluate the detection panel, interlaboratory ring tests were performed in four independent laboratories and a detection limit of 5 × 100 (AdV, HEV and RVA) and 5 × 101 (HAV, NoV) of genome equivalents in reaction was reached. The utilized rapid multiplexing technology represents an acceptably robust and sensitive method. As the results can be obtained in approximately 5 hours, its application could be useful for routine monitoring and control of viruses in food and water safety management and also in the case of viral outbreaks.

Název v anglickém jazyce

MOL-PCR and xMAP Technology: A Multiplex System for Fast Detection of Food- and Waterborne Viruses

Popis výsledku anglicky

Viruses are common causes of food- and water-borne diseases worldwide. Conventional identification of these pathogenic agents is based on cultivation, antigen detection, electron microscopy or standardized real-time polymerase chain reaction systems. Recent technological advancements in sample processing and detection methods are currently more and more focused on fast and robust analysis. Following this approach in this study, we utilized a rapid multiplexing technology, which can detect a broad spectrum of pathogenic viruses connected to food or water contamination. We have designed a new semi-quantitative magnetic bead - based multiplex panel for simultaneous detection of several targets in one reaction. The panel includes adenoviruses 40/41 (AdV), rotavirus A (RVA), norovirus (NoV), hepatitis E virus (HEV), hepatitis A virus (HAV) and target for external control of the system. To evaluate the detection panel, interlaboratory ring tests were performed in four independent laboratories and a detection limit of 5 × 100 (AdV, HEV and RVA) and 5 × 101 (HAV, NoV) of genome equivalents in reaction was reached. The utilized rapid multiplexing technology represents an acceptably robust and sensitive method. As the results can be obtained in approximately 5 hours, its application could be useful for routine monitoring and control of viruses in food and water safety management and also in the case of viral outbreaks.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10607 - Virology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

The Journal of Molecular Diagnostics

ISSN

1525-1578

e-ISSN

1943-7811

Svazek periodika

23

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

765-776

Kód UT WoS článku

000654338000011

EID výsledku v databázi Scopus

2-s2.0-85106392271