Escherichia coli Strains Producing Selected Bacteriocins Inhibit Porcine Enterotoxigenic Escherichia coli (ETEC) under both In Vitro and In Vivo Conditions

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000259" target="_blank" >RIV/00027162:_____/21:N0000259 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/21:00123081

Výsledek na webu

<a href="https://journals.asm.org/doi/10.1128/AEM.03121-20" target="_blank" >https://journals.asm.org/doi/10.1128/AEM.03121-20</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1128/AEM.03121-20" target="_blank" >10.1128/AEM.03121-20</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Escherichia coli Strains Producing Selected Bacteriocins Inhibit Porcine Enterotoxigenic Escherichia coli (ETEC) under both In Vitro and In Vivo Conditions

Popis výsledku v původním jazyce

Enterotoxigenic Escherichia coli (ETEC) and Shiga toxin-producing E. coli (STEC) strains are the causative agents of severe foodborne diseases in both humans and animals. In this study, porcine pathogenic E. coli strains (n = 277) as well as porcine commensal strains (n = 195) were tested for their susceptibility to 34 bacteriocin monoproducers to identify the most suitable bacteriocin types inhibiting porcine pathogens. Under in vitro condition, the set of pathogenic E. coli was found to be significantly more susceptible to a majority of tested bacteriocins when compared to commensal E. coli. Based on production of bacteriocins with specific activity against pathogen, three potentially probiotic commensal E. coli strains of human origin were selected. These strains were found to be able to outcompete ETEC strains expressing F4 or F18 fimbriae in liquid culture and also decrease severity and duration of diarrhea in piglets during experimental ETEC infection as well as pathogen numbers in the last day of in vivo experimentation. While the extent of the probiotic effect was different for each strain, the cocktail of all three strains showed the most pronounced beneficial effects suggesting synergy between tested E. coli strains.

Název v anglickém jazyce

Escherichia coli Strains Producing Selected Bacteriocins Inhibit Porcine Enterotoxigenic Escherichia coli (ETEC) under both In Vitro and In Vivo Conditions

Popis výsledku anglicky

Enterotoxigenic Escherichia coli (ETEC) and Shiga toxin-producing E. coli (STEC) strains are the causative agents of severe foodborne diseases in both humans and animals. In this study, porcine pathogenic E. coli strains (n = 277) as well as porcine commensal strains (n = 195) were tested for their susceptibility to 34 bacteriocin monoproducers to identify the most suitable bacteriocin types inhibiting porcine pathogens. Under in vitro condition, the set of pathogenic E. coli was found to be significantly more susceptible to a majority of tested bacteriocins when compared to commensal E. coli. Based on production of bacteriocins with specific activity against pathogen, three potentially probiotic commensal E. coli strains of human origin were selected. These strains were found to be able to outcompete ETEC strains expressing F4 or F18 fimbriae in liquid culture and also decrease severity and duration of diarrhea in piglets during experimental ETEC infection as well as pathogen numbers in the last day of in vivo experimentation. While the extent of the probiotic effect was different for each strain, the cocktail of all three strains showed the most pronounced beneficial effects suggesting synergy between tested E. coli strains.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30303 - Infectious Diseases

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

APPLIED AND ENVIRONMENTAL MICROBIOLOGY

ISSN

0099-2240

e-ISSN

1098-5336

Svazek periodika

87

Číslo periodika v rámci svazku

14

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

"e03121-20"

Kód UT WoS článku

000693249500022

EID výsledku v databázi Scopus

2-s2.0-85111784496