Detection and molecular characterisation of methicillin-resistant Staphylococcus aureus isolated from raw meat in the retail market

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000261" target="_blank" >RIV/00027162:_____/21:N0000261 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16270/21:43879684

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S2213716521001685?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2213716521001685?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jgar.2021.06.012" target="_blank" >10.1016/j.jgar.2021.06.012</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Detection and molecular characterisation of methicillin-resistant Staphylococcus aureus isolated from raw meat in the retail market

Popis výsledku v původním jazyce

This study is aimed to detect and characterize methicillin-resistant Staphylococcus aureus (MRSA) from meat retailed in the Czech Republic between 2017 and 2018. Out of 65 raw meat samples examined (poultry, beef, pork and rabbit), 23 (35.4%) were MRSA positive. Twelve positive samples originated from poultry (12/33, 36.4%), while the remaining eleven came from pork (9/9, 100%) and mix of pork and beef minced meat (2/5, 40%). All beef and rabbit meat samples were MRSA negative. In total, 36 MRSA isolates were obtained, spa typing, MLST, detection of genes encoding enterotoxins (ses), Panton-Valentine leukocidin (pvl), exfoliative toxins A, B (eta, etb), toxic shock syndrome toxin (tst), staphylokinase (sak), øSa3 prophage and antimicrobial susceptibility testing were performed. Eight spa types belonging to five different sequence types (STs) were identified. ST398 was the most frequent one (28/36, 77.8%), presenting spa types t011, t034, t2576, t4132, t588 and t899. Other LA-MRSA STs (ST9/t899, ST5/t002, ST692/t8646 or the newly described ST4034/t899) were also sporadically identified. In seven isolates (19.4%), one or more ses genes were detected, sea, seg, sei prevailed. Three isolates from turkey (ST398/t899 (n=2), ST398/t011) harboured the sak gene, the latter one harboured also the sea gene. Seven isolates from poultry harboured the øSa3 prophage as well as tetracycline resistance. None of the isolates was positive for pvl, eta or etb genes. Consequently, specific kinds of meat appear to be a possible source of MRSA, but the risk for humans is hard to define. Food animals have the potential to be a source of primary contamination of meat, therefore, surveillance of MRSA in meat and hygienic practices should be improved.

Název v anglickém jazyce

Detection and molecular characterisation of methicillin-resistant Staphylococcus aureus isolated from raw meat in the retail market

Popis výsledku anglicky

This study is aimed to detect and characterize methicillin-resistant Staphylococcus aureus (MRSA) from meat retailed in the Czech Republic between 2017 and 2018. Out of 65 raw meat samples examined (poultry, beef, pork and rabbit), 23 (35.4%) were MRSA positive. Twelve positive samples originated from poultry (12/33, 36.4%), while the remaining eleven came from pork (9/9, 100%) and mix of pork and beef minced meat (2/5, 40%). All beef and rabbit meat samples were MRSA negative. In total, 36 MRSA isolates were obtained, spa typing, MLST, detection of genes encoding enterotoxins (ses), Panton-Valentine leukocidin (pvl), exfoliative toxins A, B (eta, etb), toxic shock syndrome toxin (tst), staphylokinase (sak), øSa3 prophage and antimicrobial susceptibility testing were performed. Eight spa types belonging to five different sequence types (STs) were identified. ST398 was the most frequent one (28/36, 77.8%), presenting spa types t011, t034, t2576, t4132, t588 and t899. Other LA-MRSA STs (ST9/t899, ST5/t002, ST692/t8646 or the newly described ST4034/t899) were also sporadically identified. In seven isolates (19.4%), one or more ses genes were detected, sea, seg, sei prevailed. Three isolates from turkey (ST398/t899 (n=2), ST398/t011) harboured the sak gene, the latter one harboured also the sea gene. Seven isolates from poultry harboured the øSa3 prophage as well as tetracycline resistance. None of the isolates was positive for pvl, eta or etb genes. Consequently, specific kinds of meat appear to be a possible source of MRSA, but the risk for humans is hard to define. Food animals have the potential to be a source of primary contamination of meat, therefore, surveillance of MRSA in meat and hygienic practices should be improved.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30302 - Epidemiology

Projekt

<a href="/cs/project/ED2.1.00%2F19.0385" target="_blank" >ED2.1.00/19.0385: Rozvoj výzkumných kapacit Centra AdmireVet</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Global Antimicrobial Resistance

ISSN

2213-7165

e-ISSN

2213-7173

Svazek periodika

26

Číslo periodika v rámci svazku

September 2021

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

233-238

Kód UT WoS článku

000696999800046

EID výsledku v databázi Scopus

2-s2.0-85111330443