Gordonia species as a rare pathogen isolated from milk of dairy cows with mastitis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F22%3AN0000025" target="_blank" >RIV/00027162:_____/22:N0000025 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.nature.com/articles/s41598-022-09340-4" target="_blank" >https://www.nature.com/articles/s41598-022-09340-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-022-09340-4" target="_blank" >10.1038/s41598-022-09340-4</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Gordonia species as a rare pathogen isolated from milk of dairy cows with mastitis

Popis výsledku v původním jazyce

While Gordonia species have long been known to cause severe inflammations in human medicine, the pathogenic effects of gordonias in veterinary medicine have rarely been described. Between 2010 and 2019, we collected microorganisms of the genus Gordonia isolated from milk samples from dairy cows with mastitis. We describe the growth properties of these microorganisms, their prevalence, virulence factors and susceptibility to antimicrobial agents. From 31,534 quarter milk samples processed by standard culture methods27 isolates of Gordonia species (0.086% prevalence) were identified by a molecular phenotyping method. The isolates originated from 17 farms in 12 districts of the Czech Republic. Twenty-one isolates were tested for susceptibility to 7 antimicrobials by the disc diffusion method. Notably, 100% of these isolates were susceptible to streptomycin and neomycin, 85.6% to tetracycline and cefovecin, 76.2% to penicillin G, 47.6% to trimethoprim/sulfamethoxazole and 0% to clindamycin. The species was determined to be Gordonia paraffinivorans by whole genome sequencing for 9 isolates (from 8 farms in 7 districts). These isolates showed the highest similarity to two reference strains from the environment. In all these isolates, we identified genes encoding virulence factors, which are very similar to the genes of virulence factors expressed in Mycobacterium tuberculosis and Mycobacterium smegmatis. However, genome analysis revealed 61 unique genes in all 9 sequenced isolates.

Název v anglickém jazyce

Gordonia species as a rare pathogen isolated from milk of dairy cows with mastitis

Popis výsledku anglicky

While Gordonia species have long been known to cause severe inflammations in human medicine, the pathogenic effects of gordonias in veterinary medicine have rarely been described. Between 2010 and 2019, we collected microorganisms of the genus Gordonia isolated from milk samples from dairy cows with mastitis. We describe the growth properties of these microorganisms, their prevalence, virulence factors and susceptibility to antimicrobial agents. From 31,534 quarter milk samples processed by standard culture methods27 isolates of Gordonia species (0.086% prevalence) were identified by a molecular phenotyping method. The isolates originated from 17 farms in 12 districts of the Czech Republic. Twenty-one isolates were tested for susceptibility to 7 antimicrobials by the disc diffusion method. Notably, 100% of these isolates were susceptible to streptomycin and neomycin, 85.6% to tetracycline and cefovecin, 76.2% to penicillin G, 47.6% to trimethoprim/sulfamethoxazole and 0% to clindamycin. The species was determined to be Gordonia paraffinivorans by whole genome sequencing for 9 isolates (from 8 farms in 7 districts). These isolates showed the highest similarity to two reference strains from the environment. In all these isolates, we identified genes encoding virulence factors, which are very similar to the genes of virulence factors expressed in Mycobacterium tuberculosis and Mycobacterium smegmatis. However, genome analysis revealed 61 unique genes in all 9 sequenced isolates.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/QK22020292" target="_blank" >QK22020292: Certifikace chovů dojeného skotu dle spotřeby antimikrobik a zdraví mléčné žlázy ke snížení jejich spotřeby, racionalizaci jejich používání a tlumení rezistence k nim</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Reports

ISSN

2045-2322

e-ISSN

2045-2322

Svazek periodika

12

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

—

Kód UT WoS článku

000781187500033

EID výsledku v databázi Scopus

2-s2.0-85128217931