Association between sperm mitochondrial DNA copy number and deletion rate and industrial air pollution dynamics

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F22%3AN0000040" target="_blank" >RIV/00027162:_____/22:N0000040 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.nature.com/articles/s41598-022-12328-9" target="_blank" >https://www.nature.com/articles/s41598-022-12328-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-022-12328-9" target="_blank" >10.1038/s41598-022-12328-9</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Association between sperm mitochondrial DNA copy number and deletion rate and industrial air pollution dynamics

Popis výsledku v původním jazyce

The efects of air pollution on men’s reproductive health can be monitored by evaluating semen quality and sperm DNA damage. We used real-time PCR to analyse the efects of air pollution on sperm mitochondrial DNA copy number (mtDNAcn) and deletion (mtDNAdel) rates in semen samples collected from 54 men in two seasons with diferent levels of industrial and trafc air pollution. MtDNAdel rates were signifcantly higher following the high exposure period and were positively correlated with mtDNAcn. However, we did not fnd any diference in mtDNAcn between the two seasons. MtDNAcn was positively correlated with the DNA fragmentation index and the rates of sperm with chromatin condensation defects, previously assessed by sperm chromatin structure assay, and negatively correlated with sperm concentration, progressive motility, viability, and normal morphology. This indicates that mtDNAcn is more closely associated with male fertility than mtDNAdel rates. In contrast, mtDNAdel might be a more sensitive biomarker of air pollution exposure in urban industrial environments.

Název v anglickém jazyce

Association between sperm mitochondrial DNA copy number and deletion rate and industrial air pollution dynamics

Popis výsledku anglicky

The efects of air pollution on men’s reproductive health can be monitored by evaluating semen quality and sperm DNA damage. We used real-time PCR to analyse the efects of air pollution on sperm mitochondrial DNA copy number (mtDNAcn) and deletion (mtDNAdel) rates in semen samples collected from 54 men in two seasons with diferent levels of industrial and trafc air pollution. MtDNAdel rates were signifcantly higher following the high exposure period and were positively correlated with mtDNAcn. However, we did not fnd any diference in mtDNAcn between the two seasons. MtDNAcn was positively correlated with the DNA fragmentation index and the rates of sperm with chromatin condensation defects, previously assessed by sperm chromatin structure assay, and negatively correlated with sperm concentration, progressive motility, viability, and normal morphology. This indicates that mtDNAcn is more closely associated with male fertility than mtDNAdel rates. In contrast, mtDNAdel might be a more sensitive biomarker of air pollution exposure in urban industrial environments.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30304 - Public and environmental health

Projekt

<a href="/cs/project/EF16_019%2F0000798" target="_blank" >EF16_019/0000798: Healthy Aging in Industrial Environment HAIE</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Reports

ISSN

2045-2322

e-ISSN

2045-2322

Svazek periodika

12

Číslo periodika v rámci svazku

18 May 2022

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

—

Kód UT WoS článku

000797636300026

EID výsledku v databázi Scopus

2-s2.0-85130297086