First detection of Lake Sinai virus in the Czech Republic: a potential member of a new species

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F22%3AN0000113" target="_blank" >RIV/00027162:_____/22:N0000113 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16270/22:43880470

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s00705-022-05548-x" target="_blank" >https://link.springer.com/article/10.1007/s00705-022-05548-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00705-022-05548-x" target="_blank" >10.1007/s00705-022-05548-x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

First detection of Lake Sinai virus in the Czech Republic: a potential member of a new species

Popis výsledku v původním jazyce

Lake Sinai virus (LSV) is one of over 20 honey bee viruses. Variants of LSV have been classifed as members of two officially recognised species, Lake Sinai virus 1 and Lake Sinai virus 2. However, there are currently a limited number of whole-genome sequences, and the genetic variability of the virus indicates that additional species may need to be established. Extracted nucleic acid of 209 honey bee samples was screened by PCR for 11 honey bee viruses. LSV was the third most abundant virus (36.9% of positive samples), after Apis mellifera flamentous virus (72.2%) and deformed wing virus (52.5%). LSV-positive samples were analyzed further by PCR with primers targeting the region encoding the viral RNAdependent RNA polymerase. Subsequently, the PCR products were sequenced, and the resulting sequences were used for a frst round of phylogenetic analysis. Based on those results, several isolates were selected for whole-genome sequencing, and the complete genome sequences were used for additional phylogenetic analysis. The results indicated the presence of at least three genetically distinct groups of LSV in the Czech Republic, the most prevalent one being related to LSV 2 but too dissimilar to be considered a member of the same species. Two sequences of a major LSV strain cluster native to the Czech Republic were determined, representing the frst Czech LSV strains published to date.

Název v anglickém jazyce

First detection of Lake Sinai virus in the Czech Republic: a potential member of a new species

Popis výsledku anglicky

Lake Sinai virus (LSV) is one of over 20 honey bee viruses. Variants of LSV have been classifed as members of two officially recognised species, Lake Sinai virus 1 and Lake Sinai virus 2. However, there are currently a limited number of whole-genome sequences, and the genetic variability of the virus indicates that additional species may need to be established. Extracted nucleic acid of 209 honey bee samples was screened by PCR for 11 honey bee viruses. LSV was the third most abundant virus (36.9% of positive samples), after Apis mellifera flamentous virus (72.2%) and deformed wing virus (52.5%). LSV-positive samples were analyzed further by PCR with primers targeting the region encoding the viral RNAdependent RNA polymerase. Subsequently, the PCR products were sequenced, and the resulting sequences were used for a frst round of phylogenetic analysis. Based on those results, several isolates were selected for whole-genome sequencing, and the complete genome sequences were used for additional phylogenetic analysis. The results indicated the presence of at least three genetically distinct groups of LSV in the Czech Republic, the most prevalent one being related to LSV 2 but too dissimilar to be considered a member of the same species. Two sequences of a major LSV strain cluster native to the Czech Republic were determined, representing the frst Czech LSV strains published to date.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10607 - Virology

Projekt

<a href="/cs/project/QK1910286" target="_blank" >QK1910286: Efektivní postupy a strategie pro zvládání včelích chorob a udržitelný chov včelstev</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Archives of Virology

ISSN

0304-8608

e-ISSN

1432-8798

Svazek periodika

167

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

AT - Rakouská republika

Počet stran výsledku

10

Strana od-do

2213–2222

Kód UT WoS článku

000836132200003

EID výsledku v databázi Scopus

2-s2.0-85135294340