Genetic diversity of Lake Sinai Virus in the Czech Republic
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F22%3A43880489" target="_blank" >RIV/62157124:16270/22:43880489 - isvavai.cz</a>
Výsledek na webu
<a href="https://www.vfu.cz/files/upload/1401/KMVP%202022_Sbornik.pdf" target="_blank" >https://www.vfu.cz/files/upload/1401/KMVP%202022_Sbornik.pdf</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Genetic diversity of Lake Sinai Virus in the Czech Republic
Popis výsledku v původním jazyce
Lake Sinai virus (LSV) is a honey bee-infecting virus with two officially recognised species: LSV 1 and LSV 2. There is currently a limited number of whole-genome LSV sequences, yet the genetic variability of the virus indicates that there are more than two species in existence. Extracted nucleic acid of 209 honey bee samples were screened by PCR method for presence of LSV, which was found in 36.9% of the samples. LSV-positive samples underwent additional PCR reaction with primers targeting region coding RNA-dependent RNA polymerase of the virus. Subsequently, the PCR products were sequenced and the acquired sequences were used for first round of phylogenetic analysis. Several of the isolates were selected for whole-genome sequencing. Obtained sequences were used for additional phylogenetic analyses. The results indicate the presence of at least three genetically distinct groups of LSV in the Czech Republic. Finally, we obtained two LSV sequences via whole-genome sequencing.
Název v anglickém jazyce
Genetic diversity of Lake Sinai Virus in the Czech Republic
Popis výsledku anglicky
Lake Sinai virus (LSV) is a honey bee-infecting virus with two officially recognised species: LSV 1 and LSV 2. There is currently a limited number of whole-genome LSV sequences, yet the genetic variability of the virus indicates that there are more than two species in existence. Extracted nucleic acid of 209 honey bee samples were screened by PCR method for presence of LSV, which was found in 36.9% of the samples. LSV-positive samples underwent additional PCR reaction with primers targeting region coding RNA-dependent RNA polymerase of the virus. Subsequently, the PCR products were sequenced and the acquired sequences were used for first round of phylogenetic analysis. Several of the isolates were selected for whole-genome sequencing. Obtained sequences were used for additional phylogenetic analyses. The results indicate the presence of at least three genetically distinct groups of LSV in the Czech Republic. Finally, we obtained two LSV sequences via whole-genome sequencing.
Klasifikace
Druh
D - Stať ve sborníku
CEP obor
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OECD FORD obor
40301 - Veterinary science
Návaznosti výsledku
Projekt
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Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2022
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název statě ve sborníku
Sborník příspěvků XXIV. konference mladých vědeckých pracovníků s mezinárodní účastí
ISBN
978-80-7305-869-2
ISSN
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e-ISSN
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Počet stran výsledku
3
Strana od-do
68-70
Název nakladatele
Veterinární univerzita Brno
Místo vydání
Brno
Místo konání akce
Brno
Datum konání akce
1. 6. 2022
Typ akce podle státní příslušnosti
EUR - Evropská akce
Kód UT WoS článku
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