Development, inter-laboratory SOP evaluation and application of a molecular method for detection of Toxoplasma gondii oocysts in ready-to-eat leafy-green salads in a multicentre study in Europe

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F22%3AN0000117" target="_blank" >RIV/00027162:_____/22:N0000117 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.apicowplexa.net/index.php/bern-2021/" target="_blank" >http://www.apicowplexa.net/index.php/bern-2021/</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Development, inter-laboratory SOP evaluation and application of a molecular method for detection of Toxoplasma gondii oocysts in ready-to-eat leafy-green salads in a multicentre study in Europe

Popis výsledku v původním jazyce

Toxoplasma gondii is a zoonotic pathogen with up to 60% of acquired infections associated with foodborne transmission. Consumption of raw fresh produce contaminated with T. gondii oocysts is among the infection routes. However, the relative importance of fresh produce for human infection is underestimated as standardized detection methods are missing. The aim of this study was to develop a standard operating procedure (SOP) for molecular detection of T. gondii oocysts in leafy green salads, implement it in several laboratories, validate it by interlaboratory analysis, and apply it in a multicentre study on ready-to-eat (RTE) salad at European level ensuring delivery of robust results.

Název v anglickém jazyce

Development, inter-laboratory SOP evaluation and application of a molecular method for detection of Toxoplasma gondii oocysts in ready-to-eat leafy-green salads in a multicentre study in Europe

Popis výsledku anglicky

Toxoplasma gondii is a zoonotic pathogen with up to 60% of acquired infections associated with foodborne transmission. Consumption of raw fresh produce contaminated with T. gondii oocysts is among the infection routes. However, the relative importance of fresh produce for human infection is underestimated as standardized detection methods are missing. The aim of this study was to develop a standard operating procedure (SOP) for molecular detection of T. gondii oocysts in leafy green salads, implement it in several laboratories, validate it by interlaboratory analysis, and apply it in a multicentre study on ready-to-eat (RTE) salad at European level ensuring delivery of robust results.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30304 - Public and environmental health

Projekt

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Návaznosti

R - Projekt Ramcoveho programu EK

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů