T-zone lymphoma in dogs - immunophenotyping using multiparameter flow cytometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F22%3AN0000120" target="_blank" >RIV/00027162:_____/22:N0000120 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.solen-preview.cz/program-alergo-2022/23/" target="_blank" >https://www.solen-preview.cz/program-alergo-2022/23/</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

T-zone lymphoma in dogs - immunophenotyping using multiparameter flow cytometry

Popis výsledku v původním jazyce

T-cell lymphomas are diverse group of neoplasms with variable diagnostic features, pathophysiologies, therapeutic responses and clinical outcomes. In dogs, TLC includes indolent T-zone lymphoma (TZL) with long survival time despite generalized lymphadenopathy or high number of neoplastic cells in peripheral blood before treatment. Clinical response to chemotherapy is generally good, but in some cases, achieving of complete microscopic remission is not possible. The prevalence of TZL is estimated as 3 -14% of all canine lymphomas. TZL is characterized by the clonal expansion of T cell (CD3+) and lacking expression of the pan-leukocyte antigen CD45. Another typical feature is a high level of expression of CD 21 in most of the cases. The presence of CD4 and CD8 T-cell markers is variable. The aim of this study was to determine frequency of various immunophenotype of TZL diagnosed in Veterinary Research Institute between years 2012 and 2020 using multiparameter flow cytometry in samples of canine lymph node aspirates. In this period, 10 aspirates (4 % from 235 clinical samples) were immunophenotypically consistent with TZL (CD45-CD3+). From that, the majority of samples (5 samples) were of CD4-CD8- phenotype, followed by CD4+CD8- (3 samples) and CD4-CD8+ (2 samples). All of the CD45- T-zone cells were also stained positive for MHCII, Thy1 and CD25. In two of the TZL cases staining for CD21 was negative. Due to unique immunophenotypic features of TZL, multiparameter flow cytometry is a useful tool in the objective diagnosis and further characterization of this type of lymphoma.

Název v anglickém jazyce

T-zone lymphoma in dogs - immunophenotyping using multiparameter flow cytometry

Popis výsledku anglicky

T-cell lymphomas are diverse group of neoplasms with variable diagnostic features, pathophysiologies, therapeutic responses and clinical outcomes. In dogs, TLC includes indolent T-zone lymphoma (TZL) with long survival time despite generalized lymphadenopathy or high number of neoplastic cells in peripheral blood before treatment. Clinical response to chemotherapy is generally good, but in some cases, achieving of complete microscopic remission is not possible. The prevalence of TZL is estimated as 3 -14% of all canine lymphomas. TZL is characterized by the clonal expansion of T cell (CD3+) and lacking expression of the pan-leukocyte antigen CD45. Another typical feature is a high level of expression of CD 21 in most of the cases. The presence of CD4 and CD8 T-cell markers is variable. The aim of this study was to determine frequency of various immunophenotype of TZL diagnosed in Veterinary Research Institute between years 2012 and 2020 using multiparameter flow cytometry in samples of canine lymph node aspirates. In this period, 10 aspirates (4 % from 235 clinical samples) were immunophenotypically consistent with TZL (CD45-CD3+). From that, the majority of samples (5 samples) were of CD4-CD8- phenotype, followed by CD4+CD8- (3 samples) and CD4-CD8+ (2 samples). All of the CD45- T-zone cells were also stained positive for MHCII, Thy1 and CD25. In two of the TZL cases staining for CD21 was negative. Due to unique immunophenotypic features of TZL, multiparameter flow cytometry is a useful tool in the objective diagnosis and further characterization of this type of lymphoma.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10601 - Cell biology

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů