Flow Cytometric Characterization of Lymphoma in Dogs from the Czech Republic – A Retrospective Study

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Flow Cytometric Characterization of Lymphoma in Dogs from the Czech Republic – A Retrospective Study

Popis výsledku v původním jazyce

IV. meeting of the European Canine Lymphoma Network, Lugano, CH, 22.6.2019 - poster. Lymphoma is the most common hematologic malignancy in dogs. The diagnosis of lymphoma in dogs is based mainly on cytopathology, immunohistochemistry, flow cytometry and PCR for antigen receptor rearrangements (PARR). Flow cytometry is a very precise and accurate method to determine the immunophenotype of neoplastic cells and can provide important information regarding prognosis and response to therapy. The aim of this retrospective study was to determine the relative frequencies of various lymphoma subtypes in lymph node aspirates from 112 dogs with suspected lymphoproliferative disease using flow cytometry. Results of flow cytometry examination performed between January 2012 and December 2018 at the veterinary Research Institute were included. B-cell lymphoma was diagnosed in 70% (n=78), T-cell lymphoma in 30% (n=34) of the cases. All cases of B-cell lymphoma were positive for CD79α and CD45 and all except 4 samples were positive for CD21. Of the CD79 α+/CD45+/CD21+ tumors, 48 were MHCII+, 6 were MHCII- and 20 lymph node aspirates expressed unusual B-cell phenotype. Of the T-cell lymphoma (n=34) 4 samples each were CD4+ or CD8+, and 3 samples expressed the γδ-TCR phenotype. Four aspirates were immunophenotypically consistent with T-zone lymphoma (CD45-, CD3+, CD21+). Co-expression of CD3 with CD79 α was detected in 11 cases. In the remaining 8 samples predominance of CD3+ cells with different expression of other T-cells markers were found. In accordance with other studies, we confirmed a higher frequency of B-cell vs. T-cell lymphoma in the dog and frequent occurrence of unusual phenotypes in B-cell lymphomas.

Název v anglickém jazyce

Flow Cytometric Characterization of Lymphoma in Dogs from the Czech Republic – A Retrospective Study

Popis výsledku anglicky

IV. meeting of the European Canine Lymphoma Network, Lugano, CH, 22.6.2019 - poster. Lymphoma is the most common hematologic malignancy in dogs. The diagnosis of lymphoma in dogs is based mainly on cytopathology, immunohistochemistry, flow cytometry and PCR for antigen receptor rearrangements (PARR). Flow cytometry is a very precise and accurate method to determine the immunophenotype of neoplastic cells and can provide important information regarding prognosis and response to therapy. The aim of this retrospective study was to determine the relative frequencies of various lymphoma subtypes in lymph node aspirates from 112 dogs with suspected lymphoproliferative disease using flow cytometry. Results of flow cytometry examination performed between January 2012 and December 2018 at the veterinary Research Institute were included. B-cell lymphoma was diagnosed in 70% (n=78), T-cell lymphoma in 30% (n=34) of the cases. All cases of B-cell lymphoma were positive for CD79α and CD45 and all except 4 samples were positive for CD21. Of the CD79 α+/CD45+/CD21+ tumors, 48 were MHCII+, 6 were MHCII- and 20 lymph node aspirates expressed unusual B-cell phenotype. Of the T-cell lymphoma (n=34) 4 samples each were CD4+ or CD8+, and 3 samples expressed the γδ-TCR phenotype. Four aspirates were immunophenotypically consistent with T-zone lymphoma (CD45-, CD3+, CD21+). Co-expression of CD3 with CD79 α was detected in 11 cases. In the remaining 8 samples predominance of CD3+ cells with different expression of other T-cells markers were found. In accordance with other studies, we confirmed a higher frequency of B-cell vs. T-cell lymphoma in the dog and frequent occurrence of unusual phenotypes in B-cell lymphomas.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů