Flow Cytometry in the Diagnosis of Feline Lymphoproliferative Disorders: A Retrospective Evaluation (2020–2024)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F25%3AN0000182" target="_blank" >RIV/00027162:_____/25:N0000182 - isvavai.cz</a>

Výsledek na webu

<a href="https://amca-admin.rachel.puxdesign.cz/CSACConference/media/amca/2025/program/boa-csac2025.pdf" target="_blank" >https://amca-admin.rachel.puxdesign.cz/CSACConference/media/amca/2025/program/boa-csac2025.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Flow Cytometry in the Diagnosis of Feline Lymphoproliferative Disorders: A Retrospective Evaluation (2020–2024)

Popis výsledku v původním jazyce

[The result is a poster presentation at the "13th International Conference on Analytical Cytometry," held October 4–7, 2025, in Mikulov.] Lymphoproliferative disorders represent a significant group of oncological pathologies in cats. Accurate diagnosis is crucial for selecting appropriate treatment. Flow cytometry enables rapid and detailed immunophenotypic characterization of lymphoid cells and is essential in differentiating reactive from neoplastic processes. This retrospective study analyzed 133 samples from 97 cats suspected of lymphoproliferative disorders, examined in the Veterinary Research Institute in Brno (Czech Republic) between 2020 and 2024. Samples included lymph node, bone marrow, spleen, and peripheral blood aspirates. The most frequent finding was B-cell lymphoma with a CD79⁺CD21⁺ phenotype (18 patients), in agreement with the high incidence of B-cell lymphomas in feline populations. In seven cases, this phenotype was present in low percentages (7–27% of cells), potentially indicating early neoplastic transformation. An atypical CD79⁺CD21⁻ phenotype was observed in five patients. Mixed T- and B-cell populations with B-cell dominance were detected in 57 samples, possibly reflecting early lymphoma or reactive hyperplasia. A physiological T/B ratio with T-cell dominance was observed in 38 patients. Rare T-cell lymphomas were found in four cases, and CD4⁺CD8⁺ double-positive populations in five cases, which could suggest thymoma, although T-cell neoplasia cannot be excluded without histopathological confirmation. The study highlights the substantial heterogeneity of lymphoproliferative disorders in cats and underlines the diagnostic value of flow cytometry. It also emphasizes the necessity of complementary histopathological evaluation in ambiguous cases. This study was supported by the Ministry of Agriculture (RO0523).

Název v anglickém jazyce

Flow Cytometry in the Diagnosis of Feline Lymphoproliferative Disorders: A Retrospective Evaluation (2020–2024)

Popis výsledku anglicky

[The result is a poster presentation at the "13th International Conference on Analytical Cytometry," held October 4–7, 2025, in Mikulov.] Lymphoproliferative disorders represent a significant group of oncological pathologies in cats. Accurate diagnosis is crucial for selecting appropriate treatment. Flow cytometry enables rapid and detailed immunophenotypic characterization of lymphoid cells and is essential in differentiating reactive from neoplastic processes. This retrospective study analyzed 133 samples from 97 cats suspected of lymphoproliferative disorders, examined in the Veterinary Research Institute in Brno (Czech Republic) between 2020 and 2024. Samples included lymph node, bone marrow, spleen, and peripheral blood aspirates. The most frequent finding was B-cell lymphoma with a CD79⁺CD21⁺ phenotype (18 patients), in agreement with the high incidence of B-cell lymphomas in feline populations. In seven cases, this phenotype was present in low percentages (7–27% of cells), potentially indicating early neoplastic transformation. An atypical CD79⁺CD21⁻ phenotype was observed in five patients. Mixed T- and B-cell populations with B-cell dominance were detected in 57 samples, possibly reflecting early lymphoma or reactive hyperplasia. A physiological T/B ratio with T-cell dominance was observed in 38 patients. Rare T-cell lymphomas were found in four cases, and CD4⁺CD8⁺ double-positive populations in five cases, which could suggest thymoma, although T-cell neoplasia cannot be excluded without histopathological confirmation. The study highlights the substantial heterogeneity of lymphoproliferative disorders in cats and underlines the diagnostic value of flow cytometry. It also emphasizes the necessity of complementary histopathological evaluation in ambiguous cases. This study was supported by the Ministry of Agriculture (RO0523).

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů