Resistance of Streptococcus suis Isolates from the Czech Republic during 2018–2022

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F22%3AN0000136" target="_blank" >RIV/00027162:_____/22:N0000136 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027162:_____/23:N0000048

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Resistance of Streptococcus suis Isolates from the Czech Republic during 2018–2022

Popis výsledku v původním jazyce

A determination of minimal inhibition concentration for the monitoring of antimicrobial resistance (AMR) was carried out in 506 field isolates of Streptococcus suis, originating from sick pig on farms in the Czech Republic in the period 2018–2022. Moreover, the correlation between AMR and the serotype of the isolates was determined. A very high level of susceptibility of S. suis isolates was found to amoxicillin, in combination with clavulanic acid and sulfamethoxazole potentiated with trimethoprim. None of the tested isolates were resistant to these antimicrobial substances. Only two isolates were found to be intermediately resistant to enrofloxacin in 2020. With regard to ceftiofur, one isolate was intermediately resistant in 2020 and 2022, and two isolates were intermediately resistant in 2018 and 2021. A low level of resistance was detected to ampicillin and to florfenicol. With regard to penicillin, a medium level of resistance was detected in 2018, but a low level of resistance was found in the following years. On the contrary, a high or very high level of resistance was found to tetracycline. Using molecular and serological methods, serotype 7 (16.4%) was determined to be predominant among S. suis isolates, followed by serotypes 1/2, 2, 9, 4, 3, 1, 29, 16, and 31. Other serotypes were identified among the investigated strains either rarely (up to 10 cases) or not at all. A relatively high percentage of isolates (15.6 %) were detected as non-typeable. Dependence of resistance upon serotype assignment could not be proven in all but serotype 31, wherein all isolates (n = 17) were resistant or intermediately resistant to clindamycin, tilmycosin, tulathromycin, and tetracycline. The resistance to clindamycin and tetracycline may be related to the high consumption of these antibiotics on pig farms at present or in previous years. Macrolides (tilmicosin and tulathromycin) and tiamulin are not suitable for the treatment of streptococcal infections, but are used on pig farms to treat respiratory infections caused by gram-negative bacteria, so they were included in the study. The study was supported by the Ministry of Agriculture of the Czech Republic (MZE-RO0518) and by the National Agency for Agricultural Research (QK1810193).

Název v anglickém jazyce

Resistance of Streptococcus suis Isolates from the Czech Republic during 2018–2022

Popis výsledku anglicky

A determination of minimal inhibition concentration for the monitoring of antimicrobial resistance (AMR) was carried out in 506 field isolates of Streptococcus suis, originating from sick pig on farms in the Czech Republic in the period 2018–2022. Moreover, the correlation between AMR and the serotype of the isolates was determined. A very high level of susceptibility of S. suis isolates was found to amoxicillin, in combination with clavulanic acid and sulfamethoxazole potentiated with trimethoprim. None of the tested isolates were resistant to these antimicrobial substances. Only two isolates were found to be intermediately resistant to enrofloxacin in 2020. With regard to ceftiofur, one isolate was intermediately resistant in 2020 and 2022, and two isolates were intermediately resistant in 2018 and 2021. A low level of resistance was detected to ampicillin and to florfenicol. With regard to penicillin, a medium level of resistance was detected in 2018, but a low level of resistance was found in the following years. On the contrary, a high or very high level of resistance was found to tetracycline. Using molecular and serological methods, serotype 7 (16.4%) was determined to be predominant among S. suis isolates, followed by serotypes 1/2, 2, 9, 4, 3, 1, 29, 16, and 31. Other serotypes were identified among the investigated strains either rarely (up to 10 cases) or not at all. A relatively high percentage of isolates (15.6 %) were detected as non-typeable. Dependence of resistance upon serotype assignment could not be proven in all but serotype 31, wherein all isolates (n = 17) were resistant or intermediately resistant to clindamycin, tilmycosin, tulathromycin, and tetracycline. The resistance to clindamycin and tetracycline may be related to the high consumption of these antibiotics on pig farms at present or in previous years. Macrolides (tilmicosin and tulathromycin) and tiamulin are not suitable for the treatment of streptococcal infections, but are used on pig farms to treat respiratory infections caused by gram-negative bacteria, so they were included in the study. The study was supported by the Ministry of Agriculture of the Czech Republic (MZE-RO0518) and by the National Agency for Agricultural Research (QK1810193).

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/QK1810193" target="_blank" >QK1810193: Moderní metody diagnostiky, terapie a prevence infekcí Streptococcus suis jako nástroj pro sestavování cílených kontrolních programů v chovech prasat</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů