Antimicrobial resistance and AMR genes occurrence in Streptococcus suis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F24%3AN0000108" target="_blank" >RIV/00027162:_____/24:N0000108 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.eavld2024.org/wp-content/uploads/2024/10/Programma-DEFINITIVO-WEB.pdf" target="_blank" >https://www.eavld2024.org/wp-content/uploads/2024/10/Programma-DEFINITIVO-WEB.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Antimicrobial resistance and AMR genes occurrence in Streptococcus suis

Popis výsledku v původním jazyce

Introduction: Diseases caused by Streptococcus suis are a significant economic concern for pig farms globally and have also zoonotic potential. Antimicrobials are commonly used to treat S. suis infections, and it is therefore essential to monitor antimicrobial resistance (AMR). Materials and Methods: A total of 525 S. suis isolates were collected from diseased pigs on Czech farms between 2018 and 2022. Antimicrobial susceptibility testing (AST) was performed by determining the minimum inhibitory concentrations (MICs) using the microdilution broth method. In selected 173 isolates, genes encoding AMR were detected by searching the whole genome sequences using the ResFinder database. Results: None of the isolates tested were resistant to ceftiofur, a very high level of susceptibility of S. suis isolates was also found to amoxicillin with clavulanic acid, sulfamethoxazole potentiated with trimethoprim, enrofloxacin and florfenicol. On the contrary, high levels of resistance were found to tetracycline, clindamycin, tilmicosin, tulathromycin and tiamulin. AMR genes were predominantly present for macrolides and lincosamides (erm(B)), tetracyclines (tet(O)) and aminoglycosides (ant(6)-Ia). Discussion and Conclusion: Resistance to certain antimicrobials may be related to their high consumption in pig farms for the treatment of infections. The prevalence of AMR genes in our study is consistent with the results of phenotypic AST. However, the presence of AMR genes in the isolates did not always correspond to the AST results. This discrepancy is likely due to the presence of silent genes or, conversely, the presence of genes not identified by the available database. Any use of antimicrobials must be well justified to increase the likelihood of treatment success and prevent the spread of resistence. This work was supported by grants RO0523 and TN02000017.

Název v anglickém jazyce

Antimicrobial resistance and AMR genes occurrence in Streptococcus suis

Popis výsledku anglicky

Introduction: Diseases caused by Streptococcus suis are a significant economic concern for pig farms globally and have also zoonotic potential. Antimicrobials are commonly used to treat S. suis infections, and it is therefore essential to monitor antimicrobial resistance (AMR). Materials and Methods: A total of 525 S. suis isolates were collected from diseased pigs on Czech farms between 2018 and 2022. Antimicrobial susceptibility testing (AST) was performed by determining the minimum inhibitory concentrations (MICs) using the microdilution broth method. In selected 173 isolates, genes encoding AMR were detected by searching the whole genome sequences using the ResFinder database. Results: None of the isolates tested were resistant to ceftiofur, a very high level of susceptibility of S. suis isolates was also found to amoxicillin with clavulanic acid, sulfamethoxazole potentiated with trimethoprim, enrofloxacin and florfenicol. On the contrary, high levels of resistance were found to tetracycline, clindamycin, tilmicosin, tulathromycin and tiamulin. AMR genes were predominantly present for macrolides and lincosamides (erm(B)), tetracyclines (tet(O)) and aminoglycosides (ant(6)-Ia). Discussion and Conclusion: Resistance to certain antimicrobials may be related to their high consumption in pig farms for the treatment of infections. The prevalence of AMR genes in our study is consistent with the results of phenotypic AST. However, the presence of AMR genes in the isolates did not always correspond to the AST results. This discrepancy is likely due to the presence of silent genes or, conversely, the presence of genes not identified by the available database. Any use of antimicrobials must be well justified to increase the likelihood of treatment success and prevent the spread of resistence. This work was supported by grants RO0523 and TN02000017.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/TN02000017" target="_blank" >TN02000017: Národní Centrum Biotechnologií ve Veterinární Medicíně</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů