Enterococcus faecium-derived extracellular vesicles and their immunomodulatory effects

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F22%3AN0000178" target="_blank" >RIV/00027162:_____/22:N0000178 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.csaki.cz/odborne-akce/xxxix-sjezd-ceskych-a-slovenskych-alergologu-a-klinickych-imunologu-164" target="_blank" >https://www.csaki.cz/odborne-akce/xxxix-sjezd-ceskych-a-slovenskych-alergologu-a-klinickych-imunologu-164</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Enterococcus faecium-derived extracellular vesicles and their immunomodulatory effects

Popis výsledku v původním jazyce

Inflammatory bowel disease (IBD) is a chronic disease, often leading to serious health problems, such as chronic diarrhea, abdominal pain, or rectal bleeding. It is often accompanied by dysbiosis and enrichment in certain bacterial species (such as Enterococcus spp.). In consequence, presumably also the production and composition of bacterial membrane-derived particles, enabling intercellular communication, called extracellular vesicles (EVs), change. We hypothesize that Enterococcus faecium-derived EVs may contribute to IBD. In this work, we aim to biochemically and biophysically characterize these EVs and outline their immunomodulatory capacity. Enterococcus faecium-derived EVs size ranges between 100-150 nm in diameter and they were shown to contain LTA. First results have shown a rather pro-inflammatory action of these EVs, stimulating the formation of reactive oxygen species as well as production of pro-inflammatory cytokine TNFalpha but not immunoregulatory IL-10 by RAW264.7 macrophages. Further, the cultivation of transfected HEK293 cells with Enterococcus faecium-derived EVs suggested that the TLR2/6 heterodimer might be an important receptor for these EVs’ entrance to target cells. Our results provide a starting point for further functional studies of Enterococcus-derived EVs. These EVs seem to induce a pro-inflammatory reaction in macrophages, supposedly by interaction with the TLR2/6 receptor

Název v anglickém jazyce

Enterococcus faecium-derived extracellular vesicles and their immunomodulatory effects

Popis výsledku anglicky

Inflammatory bowel disease (IBD) is a chronic disease, often leading to serious health problems, such as chronic diarrhea, abdominal pain, or rectal bleeding. It is often accompanied by dysbiosis and enrichment in certain bacterial species (such as Enterococcus spp.). In consequence, presumably also the production and composition of bacterial membrane-derived particles, enabling intercellular communication, called extracellular vesicles (EVs), change. We hypothesize that Enterococcus faecium-derived EVs may contribute to IBD. In this work, we aim to biochemically and biophysically characterize these EVs and outline their immunomodulatory capacity. Enterococcus faecium-derived EVs size ranges between 100-150 nm in diameter and they were shown to contain LTA. First results have shown a rather pro-inflammatory action of these EVs, stimulating the formation of reactive oxygen species as well as production of pro-inflammatory cytokine TNFalpha but not immunoregulatory IL-10 by RAW264.7 macrophages. Further, the cultivation of transfected HEK293 cells with Enterococcus faecium-derived EVs suggested that the TLR2/6 heterodimer might be an important receptor for these EVs’ entrance to target cells. Our results provide a starting point for further functional studies of Enterococcus-derived EVs. These EVs seem to induce a pro-inflammatory reaction in macrophages, supposedly by interaction with the TLR2/6 receptor

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30107 - Medicinal chemistry

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů