Interaction of bacteria-derived EVs with HEK293 cell clones
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F22%3AN0000179" target="_blank" >RIV/00027162:_____/22:N0000179 - isvavai.cz</a>
Výsledek na webu
<a href="https://www.csaki.cz/odborne-akce/xxxix-sjezd-ceskych-a-slovenskych-alergologu-a-klinickych-imunologu-164" target="_blank" >https://www.csaki.cz/odborne-akce/xxxix-sjezd-ceskych-a-slovenskych-alergologu-a-klinickych-imunologu-164</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Interaction of bacteria-derived EVs with HEK293 cell clones
Popis výsledku v původním jazyce
Anti-pathogen innate immune responses, including TLRs, are triggered upon recognition of conserved pathogen-associated molecular patterns (PAMPs). TLR2 and TLR4 have been shown to play a role in sensing the surface structures of bacteria. Some of the PAMPs could be associated with the bacteria-derived extracellular vesicles (EVs) including lipoteichoic acid (LTA) and lipopolysaccharides (LPS). The aim of the study was the first insight into the interaction of Lacticaseibacillus rhamnosus- and Enterococcus faecium-derived EVs via TLRs using transfected HEK293 cells. Lacticaseibacillus rhamnosus-derived EVs consist of two populations of particles (30 40nm; 90-120nm) with a concentration of 1013 particles per ml. Enterococcus faecium-derived EVs size ranges between 100-150 nm in diameter with a concentration of 1013 particles per ml. Both bEVs were shown to contain LTA by western blotting. The functionality of transfected HEK clones was confirmed by flow cytometry and agonist receptor interaction. The cultivation of transfected HEK293 cells with Lacticaseibacillus rhamnosus-derived EVs Enterococcus faecium-derived EVs suggested the concentration-depended interaction with the TLR2/6 and TLR2/CD14 receptors. However, no interaction was detected with HEK293/TLR4-MD2-CD14 clone Lacticaseibacillus rhamnosus- and Enterococcus faecium-derived EVs seem to interact via TLR2/CD14 and TLR6/TLR2 receptors. On the other hand, no interaction was detected with HEK293/TLR4-MD2-CD14 clone.
Název v anglickém jazyce
Interaction of bacteria-derived EVs with HEK293 cell clones
Popis výsledku anglicky
Anti-pathogen innate immune responses, including TLRs, are triggered upon recognition of conserved pathogen-associated molecular patterns (PAMPs). TLR2 and TLR4 have been shown to play a role in sensing the surface structures of bacteria. Some of the PAMPs could be associated with the bacteria-derived extracellular vesicles (EVs) including lipoteichoic acid (LTA) and lipopolysaccharides (LPS). The aim of the study was the first insight into the interaction of Lacticaseibacillus rhamnosus- and Enterococcus faecium-derived EVs via TLRs using transfected HEK293 cells. Lacticaseibacillus rhamnosus-derived EVs consist of two populations of particles (30 40nm; 90-120nm) with a concentration of 1013 particles per ml. Enterococcus faecium-derived EVs size ranges between 100-150 nm in diameter with a concentration of 1013 particles per ml. Both bEVs were shown to contain LTA by western blotting. The functionality of transfected HEK clones was confirmed by flow cytometry and agonist receptor interaction. The cultivation of transfected HEK293 cells with Lacticaseibacillus rhamnosus-derived EVs Enterococcus faecium-derived EVs suggested the concentration-depended interaction with the TLR2/6 and TLR2/CD14 receptors. However, no interaction was detected with HEK293/TLR4-MD2-CD14 clone Lacticaseibacillus rhamnosus- and Enterococcus faecium-derived EVs seem to interact via TLR2/CD14 and TLR6/TLR2 receptors. On the other hand, no interaction was detected with HEK293/TLR4-MD2-CD14 clone.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
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OECD FORD obor
30107 - Medicinal chemistry
Návaznosti výsledku
Projekt
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Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2022
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů