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Interaction of bacteria-derived EVs with HEK293 cell clones

Identifikátory výsledku

  • Kód výsledku v IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F22%3AN0000179" target="_blank" >RIV/00027162:_____/22:N0000179 - isvavai.cz</a>

  • Výsledek na webu

    <a href="https://www.csaki.cz/odborne-akce/xxxix-sjezd-ceskych-a-slovenskych-alergologu-a-klinickych-imunologu-164" target="_blank" >https://www.csaki.cz/odborne-akce/xxxix-sjezd-ceskych-a-slovenskych-alergologu-a-klinickych-imunologu-164</a>

  • DOI - Digital Object Identifier

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    Interaction of bacteria-derived EVs with HEK293 cell clones

  • Popis výsledku v původním jazyce

    Anti-pathogen innate immune responses, including TLRs, are triggered upon recognition of conserved pathogen-associated molecular patterns (PAMPs). TLR2 and TLR4 have been shown to play a role in sensing the surface structures of bacteria. Some of the PAMPs could be associated with the bacteria-derived extracellular vesicles (EVs) including lipoteichoic acid (LTA) and lipopolysaccharides (LPS). The aim of the study was the first insight into the interaction of Lacticaseibacillus rhamnosus- and Enterococcus faecium-derived EVs via TLRs using transfected HEK293 cells. Lacticaseibacillus rhamnosus-derived EVs consist of two populations of particles (30 40nm; 90-120nm) with a concentration of 1013 particles per ml. Enterococcus faecium-derived EVs size ranges between 100-150 nm in diameter with a concentration of 1013 particles per ml. Both bEVs were shown to contain LTA by western blotting. The functionality of transfected HEK clones was confirmed by flow cytometry and agonist receptor interaction. The cultivation of transfected HEK293 cells with Lacticaseibacillus rhamnosus-derived EVs Enterococcus faecium-derived EVs suggested the concentration-depended interaction with the TLR2/6 and TLR2/CD14 receptors. However, no interaction was detected with HEK293/TLR4-MD2-CD14 clone Lacticaseibacillus rhamnosus- and Enterococcus faecium-derived EVs seem to interact via TLR2/CD14 and TLR6/TLR2 receptors. On the other hand, no interaction was detected with HEK293/TLR4-MD2-CD14 clone.

  • Název v anglickém jazyce

    Interaction of bacteria-derived EVs with HEK293 cell clones

  • Popis výsledku anglicky

    Anti-pathogen innate immune responses, including TLRs, are triggered upon recognition of conserved pathogen-associated molecular patterns (PAMPs). TLR2 and TLR4 have been shown to play a role in sensing the surface structures of bacteria. Some of the PAMPs could be associated with the bacteria-derived extracellular vesicles (EVs) including lipoteichoic acid (LTA) and lipopolysaccharides (LPS). The aim of the study was the first insight into the interaction of Lacticaseibacillus rhamnosus- and Enterococcus faecium-derived EVs via TLRs using transfected HEK293 cells. Lacticaseibacillus rhamnosus-derived EVs consist of two populations of particles (30 40nm; 90-120nm) with a concentration of 1013 particles per ml. Enterococcus faecium-derived EVs size ranges between 100-150 nm in diameter with a concentration of 1013 particles per ml. Both bEVs were shown to contain LTA by western blotting. The functionality of transfected HEK clones was confirmed by flow cytometry and agonist receptor interaction. The cultivation of transfected HEK293 cells with Lacticaseibacillus rhamnosus-derived EVs Enterococcus faecium-derived EVs suggested the concentration-depended interaction with the TLR2/6 and TLR2/CD14 receptors. However, no interaction was detected with HEK293/TLR4-MD2-CD14 clone Lacticaseibacillus rhamnosus- and Enterococcus faecium-derived EVs seem to interact via TLR2/CD14 and TLR6/TLR2 receptors. On the other hand, no interaction was detected with HEK293/TLR4-MD2-CD14 clone.

Klasifikace

  • Druh

    O - Ostatní výsledky

  • CEP obor

  • OECD FORD obor

    30107 - Medicinal chemistry

Návaznosti výsledku

  • Projekt

  • Návaznosti

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Ostatní

  • Rok uplatnění

    2022

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů