Lacticase/bacillus rhamnosus-derived extracellular vesicles uptake by intestinal epithelial cells in vitro

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F22%3AN0000182" target="_blank" >RIV/00027162:_____/22:N0000182 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.csaki.cz/odborne-akce/xxxix-sjezd-ceskych-a-slovenskych-alergologu-a-klinickych-imunologu-164" target="_blank" >https://www.csaki.cz/odborne-akce/xxxix-sjezd-ceskych-a-slovenskych-alergologu-a-klinickych-imunologu-164</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Lacticase/bacillus rhamnosus-derived extracellular vesicles uptake by intestinal epithelial cells in vitro

Popis výsledku v původním jazyce

Extracellular vesicles (EVs) are lipid-bilayer nanoparticles released by almost all cell types. They carry various cargoes of bioactive substances and can activate different cellular signalling pathways. Recent evidence suggests, that bEV can contribute to the development of intestine infections as well as have anti-inflammatory effects. This work aims to achieve the characterization of bEVs released by gram-positive Lacticaseibacillus rhamnosus, and visualize and describe the uptake of fluorescent-labelled bEVs into intestinal epithelial cells. Proteomics of these bEVs predicted 337 proteins in total, where the most abundant are the ones associated with environmental information. Considering the uptake, we have shown that EVs in a ratio of 103 EVs/cell are internalized by the Caco-2 cell line up to 20%. The first results obtained from the usage of inhibitors and visualization by confocal microscopy suggest, that Lacticaseibacillus rhamnosus-derived EVs are uptaken by clathrin-dependent endocytosis. These results provide information about the internalization pathways to gain insight into the mechanism of EVs uptake by intestinal epithelial cells. Lacticaseibacillus rhamnosus-derived EVs seem to be uptaken by intestinal cells up to 20% by clathrin-dependent endocytosis.

Název v anglickém jazyce

Lacticase/bacillus rhamnosus-derived extracellular vesicles uptake by intestinal epithelial cells in vitro

Popis výsledku anglicky

Extracellular vesicles (EVs) are lipid-bilayer nanoparticles released by almost all cell types. They carry various cargoes of bioactive substances and can activate different cellular signalling pathways. Recent evidence suggests, that bEV can contribute to the development of intestine infections as well as have anti-inflammatory effects. This work aims to achieve the characterization of bEVs released by gram-positive Lacticaseibacillus rhamnosus, and visualize and describe the uptake of fluorescent-labelled bEVs into intestinal epithelial cells. Proteomics of these bEVs predicted 337 proteins in total, where the most abundant are the ones associated with environmental information. Considering the uptake, we have shown that EVs in a ratio of 103 EVs/cell are internalized by the Caco-2 cell line up to 20%. The first results obtained from the usage of inhibitors and visualization by confocal microscopy suggest, that Lacticaseibacillus rhamnosus-derived EVs are uptaken by clathrin-dependent endocytosis. These results provide information about the internalization pathways to gain insight into the mechanism of EVs uptake by intestinal epithelial cells. Lacticaseibacillus rhamnosus-derived EVs seem to be uptaken by intestinal cells up to 20% by clathrin-dependent endocytosis.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30107 - Medicinal chemistry

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů