Accelular nanofibrous bilayer scaffold intrapenetrated with polydopamine network and implemented into a full-thickness wound of a white-pig model affects inflammation and healing process

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F23%3AN0000019" target="_blank" >RIV/00027162:_____/23:N0000019 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378041:_____/23:00581651 RIV/00159816:_____/23:00078250 RIV/00216224:14110/23:00130597 RIV/65269705:_____/23:00078250

Výsledek na webu

<a href="https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-023-01822-5" target="_blank" >https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-023-01822-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12951-023-01822-5" target="_blank" >10.1186/s12951-023-01822-5</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Accelular nanofibrous bilayer scaffold intrapenetrated with polydopamine network and implemented into a full-thickness wound of a white-pig model affects inflammation and healing process

Popis výsledku v původním jazyce

We fabricated a fully resorbable polydopamine (PDA) bilayered nanostructured scaffold for the treatment of defects with full loss of skin thickness. A bottom porous layer, substituting missing dermis, composed of either collagen and chitosan (Coll/Chit) or collagen and oxidized cellulose calcium salt (Coll/CaOC). A top nanofibrous layer emulating a basement membrane (BM), composed of gelatin, polycaprolactone (PCL), and CaOC. The bottom porous layer was made by a freeze-dry process, and the electrospinning process created a nanofibril mesh on the bottom porous layer. Morphological and mechanical analysis was performed using scanning electron microscopy (SEM) and RSA G2 dynamic mechanical instrument. Cytotoxicity tests, metabolic activity, proliferation, and viability of cells were performed using murine fibroblast cell lines 3T3-A31. And in vivo experiments were conducted in a porcine model, with tissue harvesting during a time period of 6 months.Polysaccharides and PDA have improved the elasticity and firmness of microfibrils, which influenced the swelling capacity and porosity of the bilayers, considering it as desirable and beneficial for restoring the function of the skin. The Coll / CaOC bilayer has shown the highest proportion of viable cells, maintained only in the presence of PDA, throughout the 14-day culture period. The expression of various cytokines in wounds has shown a non-cytotoxic effect at any stage of wound healing. It seemed that PDA supported the early stage of inflammation with expressed pro-inflammatory cytokines. At the end of healing, the expression of pro-inflammatory cytokines was comparable to that of the control samples, which means that PDA did not cause any cytotoxic or defusing effect when implanted in the skin for a longer period of time. PDA could not support the expression of pro-healing cytokines, only the expression of the examined growth factor TGF-β was higher compared to controls. This article shows that PDA at a concentration of 2mg/ml can be safely used in skin tissue applications and prepared skin grafts are ideal to meet the requirements in a proper balance between elasticity and stiffness, together with a porous architecture that allows cells to infiltrate, adhere and proliferate without cytotoxicity assessment.

Název v anglickém jazyce

Accelular nanofibrous bilayer scaffold intrapenetrated with polydopamine network and implemented into a full-thickness wound of a white-pig model affects inflammation and healing process

Popis výsledku anglicky

We fabricated a fully resorbable polydopamine (PDA) bilayered nanostructured scaffold for the treatment of defects with full loss of skin thickness. A bottom porous layer, substituting missing dermis, composed of either collagen and chitosan (Coll/Chit) or collagen and oxidized cellulose calcium salt (Coll/CaOC). A top nanofibrous layer emulating a basement membrane (BM), composed of gelatin, polycaprolactone (PCL), and CaOC. The bottom porous layer was made by a freeze-dry process, and the electrospinning process created a nanofibril mesh on the bottom porous layer. Morphological and mechanical analysis was performed using scanning electron microscopy (SEM) and RSA G2 dynamic mechanical instrument. Cytotoxicity tests, metabolic activity, proliferation, and viability of cells were performed using murine fibroblast cell lines 3T3-A31. And in vivo experiments were conducted in a porcine model, with tissue harvesting during a time period of 6 months.Polysaccharides and PDA have improved the elasticity and firmness of microfibrils, which influenced the swelling capacity and porosity of the bilayers, considering it as desirable and beneficial for restoring the function of the skin. The Coll / CaOC bilayer has shown the highest proportion of viable cells, maintained only in the presence of PDA, throughout the 14-day culture period. The expression of various cytokines in wounds has shown a non-cytotoxic effect at any stage of wound healing. It seemed that PDA supported the early stage of inflammation with expressed pro-inflammatory cytokines. At the end of healing, the expression of pro-inflammatory cytokines was comparable to that of the control samples, which means that PDA did not cause any cytotoxic or defusing effect when implanted in the skin for a longer period of time. PDA could not support the expression of pro-healing cytokines, only the expression of the examined growth factor TGF-β was higher compared to controls. This article shows that PDA at a concentration of 2mg/ml can be safely used in skin tissue applications and prepared skin grafts are ideal to meet the requirements in a proper balance between elasticity and stiffness, together with a porous architecture that allows cells to infiltrate, adhere and proliferate without cytotoxicity assessment.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Nanobiotechnology

ISSN

1477-3155

e-ISSN

1477-3155

Svazek periodika

21

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

24

Strana od-do

"80"

Kód UT WoS článku

000945497200002

EID výsledku v databázi Scopus

2-s2.0-85149501628