Residues of drugs used to treat diseases of affluence and their effects on zebrafish embryos (Danio rerio)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F23%3AN0000042" target="_blank" >RIV/00027162:_____/23:N0000042 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027162:_____/22:N0000052

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Residues of drugs used to treat diseases of affluence and their effects on zebrafish embryos (Danio rerio)

Popis výsledku v původním jazyce

In recent years, the incidence of diseases of affluence, such as diabetes mellitus, cardiovascular diseases, high blood pressure, and high cholesterol has been constantly increasing. Due to the increased prescription and consumption of drugs used to treat these diseases, concentrations of their residues are rising in the aquatic environment alarmingly. Common technologies used in wastewater treatments plants are not able to remove pharmaceutical substances completely. Thus, the residues of still biologically active substances enter surface waters and can be potentially dangerous for non-target aquatic organisms. The aim of the study was to evaluate effects of commonly used pharmaceuticals metoprolol, enalapril (at the concentration 0.1; 10; 100; 1 000, and 100 000 µg/L) metformin (at the concentration 1; 10; 100; 1 000, and 100 000 µg/L) on the embryonic stages of zebrafish (Danio rerio) using the embryonic acute toxicity test according to OECD method No 236. After four days of exposure, our results show that tested drugs influenced hatching rate at all tested concentration, which suggests that these substances might have the potential to disrupt early development of fish. Moreover, heartbeat rate was found to be affected as well. qPCR analysis indicated significant changes in the expression of genes responsible for the early development of embryos.

Název v anglickém jazyce

Residues of drugs used to treat diseases of affluence and their effects on zebrafish embryos (Danio rerio)

Popis výsledku anglicky

In recent years, the incidence of diseases of affluence, such as diabetes mellitus, cardiovascular diseases, high blood pressure, and high cholesterol has been constantly increasing. Due to the increased prescription and consumption of drugs used to treat these diseases, concentrations of their residues are rising in the aquatic environment alarmingly. Common technologies used in wastewater treatments plants are not able to remove pharmaceutical substances completely. Thus, the residues of still biologically active substances enter surface waters and can be potentially dangerous for non-target aquatic organisms. The aim of the study was to evaluate effects of commonly used pharmaceuticals metoprolol, enalapril (at the concentration 0.1; 10; 100; 1 000, and 100 000 µg/L) metformin (at the concentration 1; 10; 100; 1 000, and 100 000 µg/L) on the embryonic stages of zebrafish (Danio rerio) using the embryonic acute toxicity test according to OECD method No 236. After four days of exposure, our results show that tested drugs influenced hatching rate at all tested concentration, which suggests that these substances might have the potential to disrupt early development of fish. Moreover, heartbeat rate was found to be affected as well. qPCR analysis indicated significant changes in the expression of genes responsible for the early development of embryos.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/EF16_019%2F0000869" target="_blank" >EF16_019/0000869: Udržitelná produkce zdravých ryb v různých akvakulturních systémech - PROFISH</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů