Early immune response of two common carp breeds to koi herpesvirus infection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F23%3AN0000079" target="_blank" >RIV/00027162:_____/23:N0000079 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.eurl-fish-crustacean.eu/fish/annual-workshop" target="_blank" >https://www.eurl-fish-crustacean.eu/fish/annual-workshop</a>

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Early immune response of two common carp breeds to koi herpesvirus infection

Popis výsledku v původním jazyce

Common carp is the most economically important fish farmed in the Czech Republic. The presence of koi herpesvirus in the country was confirmed in the early 2000s and 0 to 16 outbreak sites are detected and eradicated every year since. Although the factors of resistance of various carp breeds against the virus were studied extensively, including infection trials and mapping of quantitative trait loci, evolving analytic methods present an opportunity to further characterize the reaction of the immune system of carp to the infection. Koi carp, previously confirmed as highly susceptible to KHV, and amur wild carp (confirmed resistant) were selected for this study and intraperitoneally infected with low virulence KHV strain. Viral load, expression of selected immune genes (qPCR) and changes in expression profile of proteins (mass spectrometry) were monitored in head kidney, spleen and gills of the fish 3 and 7 days post infection. Although the viral loads increased during infection, no mortalities were observed. Few significant difference were detected at the gene expression levels, but protein expression revealed earlier onset of parameters related to class I interferon signalling pathway and complement cascade in amur carp compared to common carp. This might suggest the immunological foundation of increased amur carp resistance to KHV.

Název v anglickém jazyce

Early immune response of two common carp breeds to koi herpesvirus infection

Popis výsledku anglicky

Common carp is the most economically important fish farmed in the Czech Republic. The presence of koi herpesvirus in the country was confirmed in the early 2000s and 0 to 16 outbreak sites are detected and eradicated every year since. Although the factors of resistance of various carp breeds against the virus were studied extensively, including infection trials and mapping of quantitative trait loci, evolving analytic methods present an opportunity to further characterize the reaction of the immune system of carp to the infection. Koi carp, previously confirmed as highly susceptible to KHV, and amur wild carp (confirmed resistant) were selected for this study and intraperitoneally infected with low virulence KHV strain. Viral load, expression of selected immune genes (qPCR) and changes in expression profile of proteins (mass spectrometry) were monitored in head kidney, spleen and gills of the fish 3 and 7 days post infection. Although the viral loads increased during infection, no mortalities were observed. Few significant difference were detected at the gene expression levels, but protein expression revealed earlier onset of parameters related to class I interferon signalling pathway and complement cascade in amur carp compared to common carp. This might suggest the immunological foundation of increased amur carp resistance to KHV.

Druh

O - Ostatní výsledky

CEP obor

—

OECD FORD obor

40103 - Fishery

Projekt

<a href="/cs/project/EF16_019%2F0000869" target="_blank" >EF16_019/0000869: Udržitelná produkce zdravých ryb v různých akvakulturních systémech - PROFISH</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů