Algae Prototheca as a cauative agent of bovine mastitis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F23%3AN0000092" target="_blank" >RIV/00027162:_____/23:N0000092 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Algae Prototheca as a cauative agent of bovine mastitis

Popis výsledku v původním jazyce

Background: The unicellular achlorophyllous algae Prototheca is one of the non-bacterial agents of bovine mastitis, and its prevalence in dairy cattle is increasing worldwide. Mastitis caused by Prototheca and other non-bacterial agents (yeasts or fungi) is not treatable with antibiotics, and the only defense against it is consistent prevention in dairy farms. Objectives: Non-bacterial mastitis agents are not routinely diagnosed, so there is a lack of experience in their eradication. Our work aimed to map the non-bacterial mastitis agents with a focus on the prevalence of Prototheca in the Czech Republic to propose methods of detecting these agents and measures to prevent their spread. Methods: A total of 1051 bulk tank milk samples were collected from 21 farms in the Czech Republic. For each sample, somatic cell count (SCC) was determined to indicate possible mammary gland inflammation. The presence and number of bacteria, fungi, yeasts, and Prototheca algae were determined using standard culture methods. Samples suspicious of the presence of Prototheca were identified using a two-stage real-time PCR system. Results: The average SCC was (236±139) x103/ml, and the total number of microorganisms was (129±203) x103 ml. A total of 70 (6.66%) of all bulk tank milk samples tested were found to contain at least one species of mold. At least one yeast species was present in 1013 bulk tank milk samples out of 1051, i.e., in 96.3% of cases. In 46 samples (4.37 %), one of the Prototheca species was detected, and the most prevalent was Prototheca bovis.

Název v anglickém jazyce

Algae Prototheca as a cauative agent of bovine mastitis

Popis výsledku anglicky

Background: The unicellular achlorophyllous algae Prototheca is one of the non-bacterial agents of bovine mastitis, and its prevalence in dairy cattle is increasing worldwide. Mastitis caused by Prototheca and other non-bacterial agents (yeasts or fungi) is not treatable with antibiotics, and the only defense against it is consistent prevention in dairy farms. Objectives: Non-bacterial mastitis agents are not routinely diagnosed, so there is a lack of experience in their eradication. Our work aimed to map the non-bacterial mastitis agents with a focus on the prevalence of Prototheca in the Czech Republic to propose methods of detecting these agents and measures to prevent their spread. Methods: A total of 1051 bulk tank milk samples were collected from 21 farms in the Czech Republic. For each sample, somatic cell count (SCC) was determined to indicate possible mammary gland inflammation. The presence and number of bacteria, fungi, yeasts, and Prototheca algae were determined using standard culture methods. Samples suspicious of the presence of Prototheca were identified using a two-stage real-time PCR system. Results: The average SCC was (236±139) x103/ml, and the total number of microorganisms was (129±203) x103 ml. A total of 70 (6.66%) of all bulk tank milk samples tested were found to contain at least one species of mold. At least one yeast species was present in 1013 bulk tank milk samples out of 1051, i.e., in 96.3% of cases. In 46 samples (4.37 %), one of the Prototheca species was detected, and the most prevalent was Prototheca bovis.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/QK1910092" target="_blank" >QK1910092: Nebakteriální původci mastitid a jejich vliv na kvalitu a technologické vlastnosti mléka</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů