Dietary beta-glucans supplementation effects on fish after experimental infection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F23%3AN0000137" target="_blank" >RIV/00027162:_____/23:N0000137 - isvavai.cz</a>

Výsledek na webu

<a href="https://delegate-reg.co.uk/eafp2023" target="_blank" >https://delegate-reg.co.uk/eafp2023</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Dietary beta-glucans supplementation effects on fish after experimental infection

Popis výsledku v původním jazyce

"Toulová, I., Mikulíková, I., Banďouchová, H., Minářová, H., Pojezdal, Ľ., Poštulková, E., Papežíková, I., Palíková, M., 2023. Dietary beta-glucans supplementation effects on fish after experimental infection. 21st International Conference on Diseases of Fish and Shellfish, Abstract Book, European Association of Fish Pathologists, Aberdeen, UK, 11.–14. 9. 2023, p. 214–215. Introduction: As fish production in aquaculture increases, so does the risk of diseases outbreak. In connection with current trend of antibiotics reduction, the need for substances enhancing fish resistance to various agents increases. Beta-glucans belong to these immunostimulants. Methodology: We tested the effect of yeast beta-1,3-1,6-glucans supplements in feed on the health status of common carp (Cyprinus carpio) and rainbow trout (Oncorhynchus mykiss) after exposure to Cyprinid herpesvirus 3 (CyHV-3) and Aeromonas salmonicida, respectively. The experiment on common carp included 3 groups of fish; control group and groups provided supplemented feed (beta-glucan concentration 0.1 and 0.5 %). After 3 weeks the fish were challenged with CyHV-3. The experiment on rainbow trout included 6 groups of fish; control group and groups with supplemented feed (beta-glucan concentration 0.1; 0.2; 0.5; 1 and 2 %). After 5 weeks the fish were challenged with A. salmonicida. In both tests, blood samples were taken prior the challenges and at the end of the experiments (i.e. 4 weeks after the challenge). Haematological, biochemical and immunological analyses were performed. The skin mucus was sampled for lysozyme determination and the gut microbiome was analysed in both fish species. Results: We are still processing the results of described experiments. According the first assessments it seems that beta-glucans had immunostimulatory effects in common carp. The highest titre of specific antibodies against CyHV-3 were detected in the group fed higher concentration of beta-glucans. In the same group, we also observed enhanced oxidative burst of peripheral blood phagocytes. Conclusions: The observed the enhanced specific and non-specific immune response in common carp demonstrates the positive impact of beta-glucans on fish health, confirms their suitability for further studies and preliminarily suggests their potential for use in aquaculture."

Název v anglickém jazyce

Dietary beta-glucans supplementation effects on fish after experimental infection

Popis výsledku anglicky

"Toulová, I., Mikulíková, I., Banďouchová, H., Minářová, H., Pojezdal, Ľ., Poštulková, E., Papežíková, I., Palíková, M., 2023. Dietary beta-glucans supplementation effects on fish after experimental infection. 21st International Conference on Diseases of Fish and Shellfish, Abstract Book, European Association of Fish Pathologists, Aberdeen, UK, 11.–14. 9. 2023, p. 214–215. Introduction: As fish production in aquaculture increases, so does the risk of diseases outbreak. In connection with current trend of antibiotics reduction, the need for substances enhancing fish resistance to various agents increases. Beta-glucans belong to these immunostimulants. Methodology: We tested the effect of yeast beta-1,3-1,6-glucans supplements in feed on the health status of common carp (Cyprinus carpio) and rainbow trout (Oncorhynchus mykiss) after exposure to Cyprinid herpesvirus 3 (CyHV-3) and Aeromonas salmonicida, respectively. The experiment on common carp included 3 groups of fish; control group and groups provided supplemented feed (beta-glucan concentration 0.1 and 0.5 %). After 3 weeks the fish were challenged with CyHV-3. The experiment on rainbow trout included 6 groups of fish; control group and groups with supplemented feed (beta-glucan concentration 0.1; 0.2; 0.5; 1 and 2 %). After 5 weeks the fish were challenged with A. salmonicida. In both tests, blood samples were taken prior the challenges and at the end of the experiments (i.e. 4 weeks after the challenge). Haematological, biochemical and immunological analyses were performed. The skin mucus was sampled for lysozyme determination and the gut microbiome was analysed in both fish species. Results: We are still processing the results of described experiments. According the first assessments it seems that beta-glucans had immunostimulatory effects in common carp. The highest titre of specific antibodies against CyHV-3 were detected in the group fed higher concentration of beta-glucans. In the same group, we also observed enhanced oxidative burst of peripheral blood phagocytes. Conclusions: The observed the enhanced specific and non-specific immune response in common carp demonstrates the positive impact of beta-glucans on fish health, confirms their suitability for further studies and preliminarily suggests their potential for use in aquaculture."

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40300 - Veterinary science

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů