Effect of beta-glucan additive in feed on health status of rainbow trout (Oncorhynchus mykiss) after experimental challenge with Aeromonas salmonicida

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F24%3AN0000215" target="_blank" >RIV/00027162:_____/24:N0000215 - isvavai.cz</a>

Výsledek na webu

<a href="https://eafp.org/the-1st-meeting-of-the-central-and-eastern-european-eafp-branch-2nd-announcement/" target="_blank" >https://eafp.org/the-1st-meeting-of-the-central-and-eastern-european-eafp-branch-2nd-announcement/</a>

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of beta-glucan additive in feed on health status of rainbow trout (Oncorhynchus mykiss) after experimental challenge with Aeromonas salmonicida

Popis výsledku v původním jazyce

Lepková, Z., Palíková, M., Pojezdal, Ľ., Papežíková, I., Toulová, I., Novotná, H., Blahová, J., Mikulíková, I., 2024. Effect of beta-glucan additive in feed on health status of rainbow trout (Oncorhynchus mykiss) after experimental challenge with Aeromonas salmonicida. The 1st Meeting of the Central and Eastern European EAFP Branches, Abstract Book, European Association of Fish Pathologists, Křtiny, Czech Republic, 4.–6. 12. 2024, p. 36. ISBN 978-80-7701-005-4. Along with the rise in the intensity of fish farming, the risk of infectious disease outbreaks is also increasing. The most common infections are caused by bacteria. In salmonid farming, one of the most important diseases is furunculosis caused by Aeromonas salmonicida. Antibiotic therapy is the treatment of choice for controlling bacterial infections, but this carries a number of risks for product quality, human health and the environment. Therefore, preventive methods are used nowadays to limit the occurrence of infections, such as the addition of beta-glucans to fish feed. Beta-glucans are polysaccharide derivatives of plants, algae, fungi, bacteria and yeasts, which have a positive effect especially on the non-specific immune response in fish.The aim of the experiment was to evaluate the effects of orally administered beta-glucans on juvenile rainbow trout (Oncorhynchus mykiss). The fish were divided into four groups with different concentrations of beta-glucan addition in the feed (0%, 0.2%, 0.5% and 1%). After eight weeks (a rearing part of the experiment), all groups of the fish were experimentally infected with A. salmonicida by immersion bath and monitored for another 4 weeks (an infectious part of the experiment), while they were still provided supplemented feed. Mortality, changes in fish behaviour and feed intake were assessed throughout the experiment. Both before the experimental infection and at the end of the experiment, blood was withdrawn analyses, and body surface mucus was for haematological, biochemical and immunological collected for lysozyme determination. After euthanasia of the fish, biometric parameters were determined and the intestine was removed for metagenomic analysis of the gut microbiome. A non-significant increase in phagocytic activity was revealed in all fish groups at the end of the infectious part compared to the end of the rearing part of the experiment. Only 3 parameters (Leu, MCV, MCH) changed in the haematological and biochemical examinations at the end of the feeding part. At the end of the infectious part within the biochemical parameters, statistically significant differences in (phosphorus, total protein, triacylglycerids) were observed and results of haematological examination at the end of the infectious part did not show statistically significant differences in any of the measured parameters. The level of specific antibody titre and lysozyme activity were determined with no statistically significant changes. Metagenomic analysis of the composition of the gut microbiome is still ongoing.

Název v anglickém jazyce

Effect of beta-glucan additive in feed on health status of rainbow trout (Oncorhynchus mykiss) after experimental challenge with Aeromonas salmonicida

Popis výsledku anglicky

Lepková, Z., Palíková, M., Pojezdal, Ľ., Papežíková, I., Toulová, I., Novotná, H., Blahová, J., Mikulíková, I., 2024. Effect of beta-glucan additive in feed on health status of rainbow trout (Oncorhynchus mykiss) after experimental challenge with Aeromonas salmonicida. The 1st Meeting of the Central and Eastern European EAFP Branches, Abstract Book, European Association of Fish Pathologists, Křtiny, Czech Republic, 4.–6. 12. 2024, p. 36. ISBN 978-80-7701-005-4. Along with the rise in the intensity of fish farming, the risk of infectious disease outbreaks is also increasing. The most common infections are caused by bacteria. In salmonid farming, one of the most important diseases is furunculosis caused by Aeromonas salmonicida. Antibiotic therapy is the treatment of choice for controlling bacterial infections, but this carries a number of risks for product quality, human health and the environment. Therefore, preventive methods are used nowadays to limit the occurrence of infections, such as the addition of beta-glucans to fish feed. Beta-glucans are polysaccharide derivatives of plants, algae, fungi, bacteria and yeasts, which have a positive effect especially on the non-specific immune response in fish.The aim of the experiment was to evaluate the effects of orally administered beta-glucans on juvenile rainbow trout (Oncorhynchus mykiss). The fish were divided into four groups with different concentrations of beta-glucan addition in the feed (0%, 0.2%, 0.5% and 1%). After eight weeks (a rearing part of the experiment), all groups of the fish were experimentally infected with A. salmonicida by immersion bath and monitored for another 4 weeks (an infectious part of the experiment), while they were still provided supplemented feed. Mortality, changes in fish behaviour and feed intake were assessed throughout the experiment. Both before the experimental infection and at the end of the experiment, blood was withdrawn analyses, and body surface mucus was for haematological, biochemical and immunological collected for lysozyme determination. After euthanasia of the fish, biometric parameters were determined and the intestine was removed for metagenomic analysis of the gut microbiome. A non-significant increase in phagocytic activity was revealed in all fish groups at the end of the infectious part compared to the end of the rearing part of the experiment. Only 3 parameters (Leu, MCV, MCH) changed in the haematological and biochemical examinations at the end of the feeding part. At the end of the infectious part within the biochemical parameters, statistically significant differences in (phosphorus, total protein, triacylglycerids) were observed and results of haematological examination at the end of the infectious part did not show statistically significant differences in any of the measured parameters. The level of specific antibody titre and lysozyme activity were determined with no statistically significant changes. Metagenomic analysis of the composition of the gut microbiome is still ongoing.

Druh

O - Ostatní výsledky

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/EF16_019%2F0000869" target="_blank" >EF16_019/0000869: Udržitelná produkce zdravých ryb v různých akvakulturních systémech - PROFISH</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů